Home About us Editorial board Ahead of print Current issue Search Archives Submit article Instructions Subscribe Contacts Login 
  • Users Online: 149
  • Home
  • Print this page
  • Email this page

 Table of Contents  
Year : 2021  |  Volume : 18  |  Issue : 1  |  Page : 39

The effect of adjunctive use of melatonin as a supplement on serum ferritin level in periodontal patients: A randomized, controlled trial

Department of Periodontics, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran

Date of Submission19-Oct-2019
Date of Acceptance03-May-2020
Date of Web Publication24-May-2021

Correspondence Address:
Dr. Mehrnoosh Sadighi
Department of Periodontics, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/1735-3327.316656

Rights and Permissions

Background: Ferritin is an acute-phase protein that is increased in inflammatory diseases. Melatonin has been studied due to its antioxidant effects and the elimination of free radicals. The aim of this study was to evaluate the effect of melatonin supplement as an adjunct to routine periodontal treatment on serum ferritin levels in patients with periodontitis.
Materials and Methods: Forty patients with chronic periodontitis were included in this randomized controlled clinical trial study. Twenty patients received nonsurgical periodontal treatment and twenty patients received nonsurgical periodontal treatment with adjunctive use of melatonin. Serum ferritin concentrations and periodontal parameters were recorded at baseline and 3 months after periodontal therapy. Serum ferritin level and periodontal parameters comparison at baseline and 3 months after periodontal treatment was done by Wilcoxon signed-rank test and paired sample t-test, respectively. Differences between test and control groups were evaluated by Mann–Whitney U-test for ferritin level and independent t-test for periodontal parameters, and a P ≤ 0.05 was considered statistically significant.
Results: Serum ferritin level decreased in both the control and test group in 3 months follow-up compared to baseline (P < 0.001). The reduction in ferritin levels in the test group was more than the control group, but this difference was not statistically significant (P = 0.414). Improvement in periodontal parameters were not significant between two groups (P = 0.489), but improvement rates in the test group were more than control.
Conclusion: Melatonin, as an adjunct to periodontal therapy, showed additional benefits in the reduction of serum ferritin levels and improvement of periodontal parameters.

Keywords: Chronic periodontitis, dental scaling, ferritin, inflammation melatonin

How to cite this article:
Faramarzi M, Sadighi M, Chitsazi M, Esmailzadeh M, Foladvand G. The effect of adjunctive use of melatonin as a supplement on serum ferritin level in periodontal patients: A randomized, controlled trial. Dent Res J 2021;18:39

How to cite this URL:
Faramarzi M, Sadighi M, Chitsazi M, Esmailzadeh M, Foladvand G. The effect of adjunctive use of melatonin as a supplement on serum ferritin level in periodontal patients: A randomized, controlled trial. Dent Res J [serial online] 2021 [cited 2022 May 20];18:39. Available from: https://www.drjjournal.net/text.asp?2021/18/1/39/316656

  Introduction Top

Periodontal disease is a chronic infectious disease characterized by bone and connective tissue destruction.[1] The main etiological factor in this condition is the anaerobic bacteria found in the subgingival plaque. However, the host response to bacterial products is critical in this process.[2] An important characteristic of periodontal infection is the production of free oxygen radicals by bacteria and host immune response. An imbalance between these antioxidants and pro-oxidants can lead to significant tissue degradation in periodontitis.[3] Melatonin is an endogenous hormone rhythmically generated in the pineal gland under the control of the Supra Chiasmatic Nucleus and the light/dark cycle. It plays a crucial role in many physiological processes, including blood pressure regulation, ovarian physiology, immune function, etc., Melatonin prescribed in several ways and is available as a supplement.[4] Melatonin has been studied in relation to bone remolding, osteoporosis, osseointegration of dental implants, and dentine formation. Melatonin has strong antioxidant effects that can play an important role in protecting cells against inflammatory processes and oxidative damage. After the release of melatonin into the bloodstream, it is secreted into the saliva. The proportion of plasma melatonin passing into the mouth via salivary glands seems to be almost stable, ranging from 24% to 33%.[5] It has been shown that salivary melatonin level depends on the severity of the periodontal disease. With the increasing severity of the periodontal disease, salivary melatonin level also increases. It is even suggested that salivary melatonin levels may be a diagnostic biomarker in periodontal disease. This association reflects the fact that melatonin may protect the body from bacterial invasions.[6] The protective role of melatonin in periodontal tissues can be attributed to antimicrobial properties,[7] immune regulation,[8] anti-inflammatory effects,[9] and the removal of free radicals created during the periodontal disease process.[10],[11] Studies have shown that systemic and topical administration of melatonin in rats with periodontitis induced by ligature significantly reduced the level of inflammatory enzymes compared with the control group.[12],[13] Furthermore, the administration of melatonin in diabetic rats with periodontitis reduced osteoclastic activity and bone loss.[14] Based on the results of the research regarding adjunctive use of melatonin in improving the clinical outcomes of nonsurgical periodontal treatment, treatment with melatonin as an adjunct to scaling and root planning (SRP) can improve periodontal indices compared to SRP alone.[15]

Ferritin is an acute-phase protein that increases in inflammation, autoimmune disorders, and chronic infections.[16],[17],[18] In addition to its role as an acute-phase protein, it is also involved in the storage and recycling of iron. Ferritin stores iron into non-toxic and soluble form and releases it in a controlled order way.[19] Ferritin also plays an important role in the immune response of the host, and an increased immune response leads to an increase in ferritin migration into cells to counter with infectious agents.[20] Two key factors that regulate ferritin levels are iron[21] and proinflammatory cytokines.[22] Oral infections result in a significant increase in systemic inflammatory responses that release acute phase cytokines and acute-phase proteins.[23] According to the research of Chakraborty et al.[24] Serum ferritin levels increased in patients with chronic periodontitis, and reduced to control level after periodontal treatment. In a study on hemodialysis patients who showed elevated levels of ferritin after anemia treatment, oral melatonin was prescribed for 30 days at night. After taking melatonin, ferritin level decreased significantly.[25] Melatonin acts as an effective agent in preventing the effects of iron overload that can lead to oxidative stress.[26]

Considering the effect of melatonin on periodontal disease and the relation of ferritin with this disease, the aim ofthis study was to evaluate the effect of systemic melatonin and non-surgical periodontal therapy on serum ferritin level in patients with periodontitis.

  Materials and Methods Top

Ethics and study design

This randomized clinical controlled trial was approved by the Research Ethics Committee of Tabriz-Iran University of Medical Sciences (protocol number: 1397.664) and registered with the local World Health Organization Registry Network (IRCT). Participants were selected from the patients referred for periodontal treatment to the Department of Periodontics from April 2017 to February 2018. Written informed consent was obtained from all patients before their participation in this study.


A total of 40 participants (19 males and 21women, aged 25 to 45 years) participated in this study. The included patients presented with moderate-to-severe chronic periodontitis, as defined by Armitage.[27] Patients included in the study had the following criteria: (1) Good general health; (2) ≥12 natural teeth with a minimum of three in each quadrant; (3) ≥4 mm attachment loss in about a minimum of 30%of the existing teeth; and (4) 30% ≥ teeth with probing depth (PD) of ≥5 mm and bleeding on probing. Patients with the following criteria were excluded: (1) Current or previous smokers; (2) pregnant, menopause, and lactating females; (3) Iron deficiency anemia; (4) those who had received systemic antibiotics or surgical or nonsurgical periodontal treatment within the past 12 months; and (5) those who had a history of poorly controlled diabetes, liver disease, malignancy, and radiotherapy. Patients were randomly assigned to the following two groups (control and test), including 20 patients in each group using randomization software.

Study intervention

After enrollment, all patients were trained in tooth brushing with modified bass technique and flossing twice a day. Venous blood samples were collected at baseline before recording clinical parameters. All the patients in the two groups underwent non-surgical periodontal treatment including SRP using the ultrasonic device and hand instruments. SRP was carried out by using an ultrasonic device (Various 350, NSK, Japan) and standard Gracey periodontal curettes (Hu-Fridey, Chicago, IL, USA). Periodontal treatment was performed by a single periodontist (MF). In the test group, in addition to nonsurgical periodontal treatment melatonin tablet (3 mg, once a day for 30 days) was prescribed.

All clinical evaluations were performed using a standard probe (UNC-15, Hu-Fridey Instruments, Chicago, IL, USA). Clinical measurements were conducted by a single examiner who was a trained student in this field and was blind to the study groups. Clinical measurements included PD and clinical attachment level (CAL) (were performed at four surfaces of the tooth: mesiofacial, buccal, distofacial, and lingual. The determination of examiner reproducibility was done by carrying out double clinical periodontal data recording on ten patients. Each participant was assessed twice in one appointment, and the repeat measurements were carried out masked to the first measurement. To determine the reproducibility of the examinations, each clinical parameter was recorded twice in 10 patients at a 1-h interval and in one session. Assessment of the mean difference in the scores (with 90% accuracy, k value ranging from 0.77 to 0.81) indicated that there was no systematic bias in the measurements. Gingival index (GI) was also recorded.[28] Clinical Resampling of venous blood and reevaluation of clinical parameters was done after 3 months. To avoid circadian rhythm changes, all venous blood samples were obtained early in the morning for the hematologic test.

Blood collection and analysis

Venous blood samples were taken from all participants at baseline and after 3 months by professional operators. The blood samples were transferred into sterile vacuum tubes with no anticoagulant and sent to the laboratory in <2 h. An automated analyzer (Tosoh co., Japan) was used to measure ferritin serum level using an enzymatic immunoassay technique.

Statistical analysis

All statistical analyses were performed using statistical software (SPSS, v. 17.0 Chicago: SPSS Inc) and a P value of 0.05 used as a threshold for significance. Kolmogorov–Smirnov test was used to assess the data normality distribution, which showed all periodontal parameters were distributed normally, but ferritin was found to be nonnormally distributed. Comparison of serum ferritin level and periodontal parameters at baseline and 3 months after periodontal treatment were analyzed using Wilcoxon signed-rank test and paired sample t-test, respectively. Differences between test and control groups were evaluated using Mann–Whitney U-test for ferritin level and independent t-test for periodontal parameters.

  Results Top

All participants showed a significant reduction in serum ferritin levels 3 months after periodontal treatment (P < 0.001) [Table 1]. The reduction in ferritin level in the test group was more than the control group, but this difference wasn't statistically significant (P = 0.414).
Table 1: Comparison of serum ferritin, probing depth, clinical attachment level, and gingival index at different follow-up periods in test and control groups

Click here to view

Both control and test group showed significant improvements in PD, CAL, and GI, 3 months after non-surgical periodontal treatment when compared with baseline [Table 1]. The mean PD reduction (in mm) from baseline to 3 months was 0.91 ± 0.14 for test group; 0.87 ± 0.08 for control group (P < 0.001). Improvement in this parameter was not significant between two groups (P = 0.489), but reduction rate in the test group was more than control. The mean clinical attachment gain (in mm) was 0.87 ± 0.06 at 3 months for the test group, and it was 0.83 ± 0.07 at 3 months for the control group (P < 0.001) with no significant difference between two groups (P = 0.617) but the attachment gain in the melatonin group was more than control group.

  Discussion Top

The present study was designed to evaluate the efficacy of systemic use of melatonin supplement as an adjunct to non-surgical periodontal treatment on serum ferritin levels and periodontal clinical parameters.It was shown that the serum ferritin in subjects with chronic periodontitis, after the systemic use of melatonin was lower than those that only receive nonsurgical periodontal therapy. But this reduction was not significant. According to previous studies, the serum ferritin level was higher in subjects with CP and decreased to control levels after periodontal treatment.[24] The level of ferritin, which is an acute-phase protein, rises in inflammation and chronic infections.[16] In addition to its role as an acute-phase protein, ferritin plays an important role in the storage of iron. Two effective factors regulate the level of ferritin, iron, and pro-inflammatory factors.[21],[22],[29] Periodontal disease is involved in the destruction of periodontal supporting tissues through the production of inflammatory cytokines in response to bacterial attack. Pro-inflammatory cytokines such as tumor necrosis factor (TNF), IL-1, and IL-6 have a central role in the destruction of periodontal tissues.[30],[31] Human and animal researches showed that TNF could enhance ferritin expression in muscle, fat, and other cell types and suggested a role for TNF in iron metabolism.[22] In the present study, after nonsurgical periodontal treatment and inflammation reduction, serum ferritin levels significantly decreased in both groups. This finding in our study is in agreement with Chakraborty et al.[24] showed serum ferritin levels raised in patients with chronic periodontitis and decreased to control levels after treatment. In the present study, we added the systemic administration of melatonin to periodontal routine treatment. In our research, reduction of the level of ferritin in the test group was more than the control group that this can be attributed to the use of melatonin with non-surgical periodontal treatment. Many studies have been conducted on the use of anti-inflammatory properties of melatonin in the treatment of periodontal diseases.[32],[33],[34],[35] Melatonin can enter the saliva from the bloodstream and then reach the oral cavity. But its salivary concentration is one-third of its concentration in the blood 5. The anti-inflammatory properties of melatonin are related to its ability to remove oxygen radicals released from the inflammatory process.[32] Cutando et al.[5] showed the salivary levels of melatonin are associated with the severity of the periodontal disease. As the severity of periodontitis increased, the salivary level of melatonin increases, and this implies that melatonin protects the body against bacterial invasion. Hence, melatonin may be effective in the treatment of periodontal diseases. In this study, improvement of the results of non-surgical periodontal treatment may be related to the adjective use of melatonin.

The effect of melatonin on the ferritin levels in our study can be justified by the study of Amer et al.[26] They evaluated the protective effect of melatonin on iron overload and its toxic prosperities on the brain and testis of male rats. Despite the importance of iron in the body, imbalance in its amount can be associated with hemochromatosis and neuronal damage as a result of producing free radicals.[33] In the study of Amer et al.[26] the administration of ferrous sulfate to rats increased the level of serum iron and ferritin, and iron concentration elevated in the brain and testis. An increase in serum ferritin levels may be one of the early clinical signs of iron overload, in which iron is stored in ferritin inside the cells. The results of this study suggested that melatonin might reduce the production of destructive free radicals produced by iron toxicity. This research demonstrated serum iron, ferritin in the melatonin-treated group were normalized significantly as compared with the iron group.[26] These findings were agreement with Othman et al.[34] who suggested that melatonin can prevent oxidation of iron-binding proteins. In one conclusion, the chelation of iron ions by melatonin is important in the anti-free radical effects of melatonin, which prevents or at least decreases iron-mediated tissue oxidative stress.

In this present study, we found that this co-treatment in patients with moderate to severe periodontitis causedmore decrease in CAL, PD, and GI than those patients who received only non-surgical therapy-. This further improvement can be attributed to the systemic use of melatonin. In a study by Chitsazi et al.,[15] they evaluated the combined use of vitamin C and melatonin supplements with non-surgical periodontal therapy. The results of this study on the improvement of periodontal clinical parameters in the melatonin group relative to the scaling alone were in agreement with the present study. Cutando et al.[35] were observed similar results in diabetic patients, and there was a significant improvement in clinical parameters (PD, CAL, GI). The more improvements in the study of Cutando et al.[35] compared to the present study, can be attributed to the local use of melatonin. The use of the therapeutic effects of melatonin has documented by animal studies and clinical trials.[35],[36],[37],[38] These studies suggested the adjunctive use of melatonin in the treatment of periodontal diseases can have a beneficial effect.

Small sample size and short-term follow-up periods were our research limitations. It is recommended that long-term longitudinal studies with a large sample size be performed to determine whether serum ferritin levels can be used as a marker for diagnosis of periodontal disease or as marker for treatment response evaluation. Despite several studies that recommend the use of melatonin in dentistry, there are still some constraints that are needed to be overcome. Studies on antimicrobial effects of melatonin and the effects of this supplement on periodontal inflammatory cytokines are also recommended.

  Conclusion Top

According to the present study results, improvement in all periodontal parameters along with a reduction in serum ferritin levels was observed 3 months after periodontal therapy. However, the improvement in the melatonin group was more than the scaling group alone. By taking the data of this study and previous information can be concluded that inflammation may increase ferritin expression in serum of patients with chronic periodontitis; other factors can also participate in increasing serum ferritin, including bacterial load and virulence factors of the bacteria.

Financial support and sponsorship


Conflicts of interest

The authors of this manuscript declare that they have no conflicts of interest, real or perceived, financial or nonfinancial in this article.

  References Top

Reiter RJ, Tan DX, Poeggeler B, Menendez-Pelaez A, Chen LD, Saarela S. Melatonin as a free radical scavenger: Implications for aging and age-related diseasesa. Ann N Y Acad Sci 1994;719:1-12.  Back to cited text no. 1
Loesche WJ, Grossman NS. Periodontal disease as a specific, albeit chronic, infection: Diagnosis and treatment. Clin Microbiol Rev 2001;14:727-52.  Back to cited text no. 2
Cutando A, Gómez-Moreno G, Arana C, Acuña-Castroviejo D, Reiter RJ. Melatonin: Potential functions in the oral cavity. J Periodontol 2007;78:1094-102.  Back to cited text no. 3
Liu J, Huang F, He HW. Melatonin effects on hard tissues: Bone and tooth. Int J Mol Sci 2013;14:10063-74.  Back to cited text no. 4
Cutando A, Galindo P, Gómez-Moreno G, Arana C, Bolaños J, Acuña-Castroviejo D, et al. Relationship between salivary melatonin and severity of periodontal disease. J Periodontol 2006;77:1533-8.  Back to cited text no. 5
Patel MD, Shakir QJ, Shetty A. Interrelationship between chronic periodontitis and anemia: A 6-month follow-up study. J Indian Soc Periodontol 2014;18:19-25.  Back to cited text no. 6
[PUBMED]  [Full text]  
Poon AM, Liu ZM, Pang CS, Brown GM, Pang SF. Evidence for a direct action of melatonin on the immune system. Biol Signals 1994;3:107-17.  Back to cited text no. 7
Reiter RJ, Calvo JR, Karbownik M, Qi W, Tan DX. Melatonin and its relation to the immune system and inflammation. Ann N Y Acad Sci 2000;917:376-86.  Back to cited text no. 8
Permuy M, López-Peña M, González-Cantalapiedra A, Muñoz F. Melatonin: A Review of Its Potential Functions and Effects on Dental Diseases. Int J Mol Sci 2017;18:4:865.  Back to cited text no. 9
Salvi GE, Beck JD, Offenbacher S. PGE2, IL-1 β, and TNF-α responses in diabetics as modifiers of periodontal disease expression. Ann Periodontol 1998;3:40-50.  Back to cited text no. 10
Palaoğlu Ö, Beşkonaklı E. Pineal gland and aging. Turk Geriatri Derg 1998;1:13-8.  Back to cited text no. 11
Gulle K, Akpolat M, Kurcer Z, Cengiz M, Baba F, Acikgoz S. Multi-organ injuries caused by lipopolysaccharide-induced periodontal inflammation in rats: Role of melatonin. J Periodontol Res 2014;49:736-41.  Back to cited text no. 12
Arabacı T, Kermen E, Özkanlar S, Köse O, Kara A, Kızıldağ A, et al. Therapeutic effects of melatonin on alveolar bone resorption after experimental periodontitis in rats: A biochemical and immunohistochemical study. J Periodontol 2015;86:874-81.  Back to cited text no. 13
Yuce HB, Karatas O, Turkal HA, Gorgun EP, Ocakli S, Benli I, et al. The effect of melatonin on bone loss, diabetic control, and apoptosis in rats with diabetes with ligature-induced periodontitis. JOP 2016;87:e35-43.  Back to cited text no. 14
Chitsazi M, Faramarzie M, Sadighi M, Shirmohammadi A, Hashemzadeh A. Effects of adjective use of melatonin and vitamin C in the treatment of chronic periodontitis: A randomized clinical trial. J Dent Res Dent Clin Dent Prospects 2017;11:236-40.  Back to cited text no. 15
Uppal SS, Al-Mutairi M, Hayat S, Abraham M, Malaviya A. Ten years of clinical experience with adult onset Still's disease: Is the outcome improving? Clin Rheumatol 2007;26:1055-60.  Back to cited text no. 16
Da Costa R, Szyper-Kravitz M, Szekanecz Z, Csépány T, Dankó K, Shapira Y, et al. Ferritin and prolactin levels in multiple sclerosis. Isr Med Assoc J 2011;13:91-5.  Back to cited text no. 17
Blake DR, Bacon PA, Eastham EJ, Brigham K. Synovial fluid ferritin in rheumatoid arthritis. Br Med J 1980;281:715-6.  Back to cited text no. 18
Theil EC. Ferritin: Structure, gene regulation, and cellular function in animals, plants, and microorganisms. Annu Rev Biochem 1987;56:289-315.  Back to cited text no. 19
Chow JK, Werner BG, Ruthazer R, Snydman DR. Increased serum iron levels and infectious complications after liver transplantation. Clin Infect Dis 2010;51:e16-23.  Back to cited text no. 20
Torti FM, Torti SV. Regulation of ferritin genes and protein. Blood 2002;99:3505-16.  Back to cited text no. 21
Torti SV, Kwak EL, Miller SC, Miller LL, Ringold GM, Myambo KB, et al. The molecular cloning and characterization of murine ferritin heavy chain, a tumor necrosis factor-inducible gene. J Biol Chem 1988;263:12638-44.  Back to cited text no. 22
Becerik S, Öztürk VÖ, Atmaca H, Atilla G, Emingil G. Gingival crevicular fluid and plasma acute-phase cytokine levels in different periodontal diseases. JOP 2012;83:1304-13.  Back to cited text no. 23
Chakraborty S, Tewari S, Sharma RK, Narula SC. Effect of non-surgical periodontal therapy on serum ferritin levels: An interventional study. JOP 2014;85:688-96.  Back to cited text no. 24
Labonia W, Rubio D, Arias C. Melatonin corrects reticuloendothelial blockade and iron status in haemodialysed patients. Nephrology (Carlton) 2005;10:583-7.  Back to cited text no. 25
Amer MA, Othman AI, Sedki D. Assessment the role of melatonin in iron toxicity in male rats. Can J Clin Nutr 2014;2:22-40.  Back to cited text no. 26
Armitage GC. Development of a classification system for periodontal diseases and conditions. Ann Periodontol 1999;4:1-6.  Back to cited text no. 27
Loe H, Silness J. Periodontal disease in pregnancy. I. prevalence and severity. Acta Odontol Scand 1963;21:533-51.  Back to cited text no. 28
Muntané-Relat J, Ourlin JC, Domergue J, Maurel P. Differential effects of cytokines on the inducible expression of CYP1A1, CYP1A2, and CYP3A4 in human hepatocytes in primary culture. Hepatology 1995;22:1143-53.  Back to cited text no. 29
Graves DT. The potential role of chemokines and inflammatory cytokines in periodontal disease progression. Clin Infect Dis 1999;28:482-90.  Back to cited text no. 30
Page RC. The role of inflammatory mediators in the pathogenesis of periodontal disease. J Periodontal Res 1991;26:230-42.  Back to cited text no. 31
Manchester LC, Coto-Montes A, Boga JA, Andersen LP, Zhou Z, Galano A, et al. Melatonin: An ancient molecule that makes oxygen metabolically tolerable. J Pineal Res 2015;59:403-19.  Back to cited text no. 32
Altamura S, Muckenthaler MU. Iron toxicity in diseases of aging: Alzheimer's disease, Parkinson's disease and atherosclerosis. J Alzheimers Dis 2009;16:879-95.  Back to cited text no. 33
Othman AI, El-Missiry MA, Amer MA, Arafa M. Melatonin controls oxidative stress and modulates iron, ferritin, and transferrin levels in adriamycin treated rats. Life Sci 2008;83:563-8.  Back to cited text no. 34
Cutando A, López-Valverde A, de Diego RG, de Vicente J, Reiter R, Fernández MH, et al. Effect of topical application of melatonin to the gingiva on salivary osteoprotegerin, RANKL and melatonin levels in patients with diabetes and periodontal disease. Odontology 2014;102:290-6.  Back to cited text no. 35
Gómez-Florit M, Ramis JM, Monjo M. Anti-fibrotic and anti-inflammatory properties of melatonin on human gingival fibroblasts in vitro. Biochem Pharmacol 2013;86:1784-90.  Back to cited text no. 36
Cutando A, López-Valverde A, Gómez-de-Diego R, Arias-Santiago S, de Vicente-Jiménez J. Effect of gingival application of melatonin on alkaline and acid phosphatase, osteopontin and osteocalcin in patients with diabetes and periodontal disease. Med Oral Patol Oral Cir Bucal 2013;18:e657-63.  Back to cited text no. 37
Cutando A, Montero J, Gómez-de Diego R, Ferrera MJ, Lopez-Valverde A. Effect of topical application of melatonin on serum levels of C-reactive protein (CRP), interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) in patients with type 1 or type 2 diabetes and periodontal disease. J Clin Exp Dent 2015;7:e628-33.  Back to cited text no. 38


  [Table 1]


Similar in PUBMED
 Related articles
Access Statistics
Email Alert *
Add to My List *
* Registration required (free)  

  In this article
Materials and Me...
Article Tables

 Article Access Statistics
    PDF Downloaded270    
    Comments [Add]    

Recommend this journal