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Regeneration of dentin-pulp complex by using dental pulp stem cells in dog
Fatemeh Dehghani Nazhvani1, Setareh Kazempour2, Seyed-Mojtaba Hosseini3, Ali Dehghani Nazhvani4, Pardis Haddadi5
1 Bone and Joint Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
2 Research Committee, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran
3 Research Committee, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
4 Department of Oral and Maxillofacial Pathology, Biomaterials Research Center, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran
5 Department of Periodontology, School of Dentistry, Lorestan University of Medical Sciences, Khorramabad, Iran
Correspondence Address:
Dr. Ali Dehghani Nazhvani
Department of Oral and Maxillofacial Pathology, Biomaterials Research Center, School of Dentistry, Shiraz University of Medical Sciences, Shiraz
Iran
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/1735-3327.328754
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Introduction: Although missing tooth is not life-threatening, it affects the quality of daily life. Stem cells have emerged as an important player in the generation and maintenance of many tissues. The role of scaffolds has changed from a passive carrier to a bioactive matrix, which can be used to induce cellular behavior. The aim of this study was to determine the possibility of regeneration of dentin-pulp complex with dental pulp stem cells (DPSCs) in an animal model.
Materials and Methods: In this animal study after extraction of DPSCs and cultivation, 10 types of scaffolds were made by using platelet-rich plasma (PRP), cancellous bone, and collagen pad. They were inserted in different parts of the dog's mouth. After the 4th month, the area was operated, and the scaffolds were removed.
Results: Microscopic examination revealed no sign of cell differentiation and formation of new structures in those models which used collagen scaffolds. However, the dentin-pulp complex emerged in models that the combination of bone scaffolds and PRP or stem cells was used.
Conclusion: Using bone scaffolds in combination with PRP or DPSCs to regenerate dentin-pulp complex in dog helped odontoblastic and pulpal differentiation as well as the formation of predentin and tubular dentin.
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