Home About us Editorial board Ahead of print Current issue Search Archives Submit article Instructions Subscribe Contacts Login 
  • Users Online: 493
  • Home
  • Print this page
  • Email this page
Year : 2021  |  Volume : 18  |  Issue : 1  |  Page : 8

Biocompatibility of several colloidal solutions containing nanoparticles on human gingival fibroblasts

1 Dental Research Center, School of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran
2 Student Research Committee, School of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran

Correspondence Address:
Prof. Farzaneh Ahrari
Dental Research Center, School of Dentistry, Mashhad University of Medical Sciences, Vakilabad Blvd, Mashhad
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/1735-3327.310037

Rights and Permissions

Background: There is little information concerning the biocompatibility of mouthwashes containing metal nanoparticles. This study was conducted to assess the biocompatibility of colloidal solutions containing zinc oxide (ZnO), copper oxide (CuO), titanium dioxide (TiO2), and silver (Ag) nanoparticles compared with chlorhexidine (CHX) in a culture of human gingival fibroblasts (HGFs). Materials and Methods: This was an in vitro, experimental study. Nanoparticles, including ZnO, CuO, TiO2, and Ag, were purchased and added to a water-based solution to produce mouthwashes. The colloidal solutions and CHX were prepared at the minimum inhibitory concentration (MIC) against Streptococcus mutans and Streptococcus sanguis. Cytotoxicity was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay on HGFs at the concentrations of MIC, 0.1 MIC, and 0.01 MIC. To determine apoptosis, DNA fragmentation was assessed as “sub-G1” peak on DNA content histogram. The data were analyzed using repeated measures analysis at P < 0.05. Results: At all concentrations, the highest and lowest mean of cell viability was related to TiO2 and ZnO groups, respectively. At MIC, the mean cell viability was significantly greater in the TiO2 group than the other groups (except the Ag group) (P < 0.05). At the concentration of 0.01 MIC, the mean cell viability in the colloidal solution containing ZnO nanoparticles was significantly lower than the other solutions (P < 0.05). The CHX and CuO-containing solution displayed the highest rate of apoptosis among the groups. Conclusion: The TiO2-containing solution can be suggested as a suitable alternative to CHX to provide antiseptic effects with minimal toxicity.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded159    
    Comments [Add]    

Recommend this journal