Comparison of antimicrobial effect of several decontaminating methods on contaminated Titanium discs
Reza Birang1, Kourosh Shakerian2, Ehsan Birang3, Tahmineh Narimani4, Narges Naghsh5, Reza Fekrazad6
1 Department of Periodontology, Dental Research Center, Dental Research Institute, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran
2 Department of Periodontology, School of Dentistry, Shahrekord University of Medical Sciences, Shahrekord, Iran
3 Periodontist, School of Dentistry, International Campus, Iran University of Medical Sciences, Tehran, Iran
4 Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
5 Department of Periodontology, Dental Implants Research Center, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran
6 Department of Periodontology, Dental Faculty-Laser Research Center in Medical Sciences, AJA University of Medical Sciences, Tehran, Iran
Dr. Kourosh Shakerian
Department of Periodontology, Dental School, Shahrekord University of Medical Sciences, Shahrekord
Source of Support: None, Conflict of Interest: None
Background: Decontaminating the implant surface, exposed to bacterial biofilm, is a concern in the treatment of peri-implant inflammatory disease. The aim of this study was to compare the effect of several methods on reduction of the bacterial load, colonized on the surfaces of titanium discs.
Materials and Methods: In this in vivo study, seven titanium discs with Sandblasted, Large-grit, acid-etched (SLA) surface were placed in the mouth of each of ten patients with chronic periodontitis by an intra-oral maxillary splint for 24 h. In each patient, the contaminated discs, except for the negative control ones, were randomly treated by one of the six antiseptic methods including sterile normal saline, plastic curette, air polisher, hydrogen peroxide, 980 nm diode laser, and Er-YAG laser. A spectrophotometer was used to measure Optical Density (OD) in case of aerobic microorganisms. Colony-Forming Units (CFUs) were used for anaerobic bacteria. Data were analyzed through Kruskal–Wallis and Mann–Whitney Tests at a significance level of α =0.05 by SPSS software.
Results: Statistical analysis showed a significant decrease in OD of aerobic bacteria among the seven groups during a 0–24 h time interval (P < 0.001). Furthermore, these tests showed a significant difference in the CFU (P < 0.001) for anaerobic bacteria after 48 h.
Conclusion: The results of this study showed that all of the adopted methods significantly reduced microbial colonies on the surfaces of titanium discs with SLA surface. Er: YAG laser and normal saline had the highest and the lowest effects, respectively.