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Case Report:
Skin and mucosal ischemia as a complication after inferior alveolar nerve block
Pedro Christian Aravena, Camila Valeria, Nicolás Nuñez, Francisco Perez-Rojas, Cesar Coronado
Dent Res J
2016, 13:560 (29 December 2016)
DOI
:10.4103/1735-3327.197040
PMID
:28182074
The anesthetic block of the inferior alveolar nerve (IAN) is one of the most common techniques used in dental practice. The local complications are due to the failures on the anesthetic block or to anatomic variations in the tap site such as intravascular injection, skin ischemia and ocular problems. The aim of this article is to present a case and discuss the causes of itching and burning sensation, blanching, pain and face ischemia in the oral cavity during the IAN block.
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Original Article:
Correlation between dental caries experience and mutans streptococci counts by microbial and molecular (polymerase chain reaction) assay using saliva as microbial risk indicator
SG Damle, Ashish Loomba, Abhishek Dhindsa, Ashu Loomba, Vikas Beniwal
Dent Res J
2016, 13:552 (29 December 2016)
DOI
:10.4103/1735-3327.197035
PMID
:28182053
Background:
The aim of this study was to assess the relationships of quantitative salivary levels of mutans streptococci (MS) in children, aged 3–6 years and 12–15 years, exhibiting variable patterns of caries activity, and to compare the association of MS in saliva using microbial and molecular (polymerase chain reaction [PCR]) assay.
Materials and Methods:
In this cross-sectional observational study, eighty children were included, forty children each in Group I (3–6 years) and Group II (12–15 years). Children were further divided into two subgroups (Group IC, INC and Group IIC, IINC) based on their dental caries status. Saliva samples were collected and plated onto Mitis Salivarius-Bacitracin agar plates. After detection of MS, DNA was isolated and purified, and MS were evaluated using the PCR and AP-PCR.
Results:
Of the selected 80 children, 42 were male and 38 were female. In Group IC, the mean colony count was 2.27 ± 0.54 × 10
5
CFU/ml, and in Group INC, the mean colony was found to be 1.61 ± 0.54 × 10
5
CFU/ml. In Group II, where mean colony count of 3.31 ± 0.85 × 10
5
CFU/ml and 2.44 ± 0.54 × 10
5
CFU/ml was observed in Group IIC and Group IINC, respectively.
Conclusion:
The mean colony count increased with increasing age and was also more in children with dental caries. Based on the matrices generated by the PCR analysis using coefficient slipped-strand mispairing, wide range of genetic diversity was seen in cases of children with and without clinically detectable caries.
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Original Article:
Physical activity among dental health professionals in Hyderabad City: A questionnaire survey
Adepu Srilatha, Dolar Doshi, M Padma Reddy, Suhas Kulkarni, B Srikanth Reddy, Sahithi Reddy
Dent Res J
2016, 13:544 (29 December 2016)
DOI
:10.4103/1735-3327.197038
PMID
:28182058
Background:
To assess and compare physical activity based on age, gender, marital status, education, and employment among dental health professionals in Hyderabad City, India.
Materials and Methods:
A cross-sectional questionnaire study was conducted among dentists and they were personally interviewed by a single trained interviewer. The frequency (in days) and time (in minutes) spent in doing vigorous- and moderate-intensity activity in a typical week in three domains and sedentary behavior were assessed using 16-item Global Physical Activity Questionnaire. Metabolic equivalents (METs) were used to express the intensity of physical activities.
Results:
Overall, 60.7% of the 313 respondents were physically active, with activity at work and commuting activity were the main contributors of physical activity. With increasing age, there was a decrease in physical activity with more sedentary behavior, professionals in age group of 21–30 years, who were single, those with Bachelor's Degree and those in teaching field performed activity at work, commuting, and recreational activity for more minutes with overall high MET minutes per week and least sedentary behavior.
Conclusion:
The prevalence of physical activity was high among dental health professionals.
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Original Article:
The most painful site of maxillary anterior infiltrations
Roohollah Sharifi, Hesamedin Nazari, Peik Bolourchi, Saber Khazaei, Masoud Parirokh
Dent Res J
2016, 13:539 (29 December 2016)
DOI
:10.4103/1735-3327.197030
PMID
:28182062
Background:
The purpose of this study was to determine the most painful site of infiltration injection in the anterior part of maxilla.
Materials and Methods:
This single-blinded clinical trial was conducted on thirty healthy volunteers. The participants received three maxillary infiltrations injected at the region of central and lateral incisors as well as canines at three separated appointments with a 2-week interval. The outcome variable was pain that measured immediately after needle insertion (time = 0) and during injection of anesthetic solution in 5, 30, and 55 s by a visual analog scale. Data were analyzed by SPSS software version 16 using Friedman test.
Results:
There was no statistically significant difference in terms of needle insertion pain and during injection (time = 0, 5, 30, and 55 s) (
P
= 0.319,
P
= 0.849,
P
= 0.627, and
P
= 0.939, respectively) in the three injection sites.
Conclusion:
The pain intensity of infiltration was not associated with injection sites in the anterior maxilla.
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Original Article:
Attitude toward Public Health Dentistry as a career among dental students in Odisha: A Cross-sectional study
Nupur Sharma, Kittu Jain, Soumik Kabasi
Dent Res J
2016, 13:532 (29 December 2016)
DOI
:10.4103/1735-3327.197042
PMID
:28182073
Background:
Knowledge of dental students' expectations of their profession as well as their attitudes to study a particular specialty of dentistry is of great importance. These attitudes and expectations make studying dentistry meaningful to dental students and society and understanding these factors facilitate workforce planning in the dental sector The aim of the study was to assess the attitude of dental students towards considering Public Health Dentistry as their future career.
Materials and Methods:
A questionnaire-based, cross-sectional survey was conducted, which included the 3
rd
year, 4
th
fourth year and dental interns studying in the State of Odisha. It consisted of 27 questions that were graded on 5-point Likert scale. The responses for the attitude questions toward selecting Public Health Dentistry for postgraduation were categorized into three factors, which are a negative attitude (includes score 0–21), neutral attitude (score 22–44), and positive attitude (score 45–64). Differences between groups were examined using Chi-square test for proportions. The level of statistical significance was set at
P
< 0.05.
Results:
Among 886 respondents, 302 (34.08%) were males and 584 (65.91%) were females. One-third (33.52%) of them had a positive attitude toward selecting public health dentistry as their future career, and nearly two-third of them (58.23%) had neutral attitude, with very few students having a negative attitude (8.23%) toward the specialty for pursuing postgraduation.
Conclusion:
Respondents had a considerable amount of interest in pursuing postgraduation in this specialty. Efforts should be intensified, both by the dental council and by the dental colleges, to develop this specialty, keeping in mind the increasing attitude of dental undergraduates toward it.
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Original Article:
Comparative evaluation of canal cleaning ability of various rotary endodontic filesin apical third: A scanning electron microscopic study
Ganesh Ranganath Jadhav, Priya Mittal, Anish Kulkarni, Shibli Syed, Ravikiran Bagul, Saina Elahi, Dheeraj Kalra
Dent Res J
2016, 13:508 (29 December 2016)
DOI
:10.4103/1735-3327.197031
PMID
:28182065
Background:
The purpose of this study was to evaluate the canal cleaning ability of three novel endodontic rotary instruments and compare with ProTaper files as a control in apical third of root canals under scanning electron microscopy (SEM).
Materials and Methods:
Eighty freshly extracted mandibular premolars were selected according to inclusion criteria. Buccal cusp tips were ground to ensure having a flat coronal reference point with a total tooth length of 16 mm for all samples. Teeth were divided equally into four groups: Group I (ProTaper group), Group II (ProTaper next group), Group III (variable taper group), and Group IV (self-adjusting file [SAF] group). Using SEM, the dentinal surfaces were observed and rated at apical thirds with a magnification of ×1000 for the presence/absence of smear layer and debris. Descriptive analysis was performed, and analysis of variance with Bonferroni
post hoc
test was carried out for comparison between the groups, at a significance level of 0.05.
Results:
There was statistically significant difference between Group II and Group IV for debris (
P
= 0.047) and smear layer (
P
= 0.037).
Conclusion:
In apical third of root canal, SAF showed statistically significant canal cleaning ability due to combined effect of continuous streaming irrigation with effectively replacing the irrigant from the apical portion of the root canal, irrigants activation through the creation of turbulence, and its self-adapting design to root canal anatomy with a scrubbing motion on the canal walls.
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Original Article:
Evaluation of the effects of two different bone resorption inhibitors on osteoclast numbers and activity: An animal study
Narges Naghsh, Sayed Mohammad Razavi, Mohsen Minaiyan, Mohammad Shahabooei, Reza Birang, Parichehr Behfarnia, Samira Hajisadeghi
Dent Res J
2016, 13:500 (29 December 2016)
DOI
:10.4103/1735-3327.197034
PMID
:28182072
Background:
The aim of this study was to evaluate the effects of bone resorption inhibitors, doxycycline (DOX) and erythromycin (EM), on osseous wound healing in rat alveolar socket.
Materials and Methods:
In this randomized controlled trial, 45 8–10-week-old male Wistar rats had their maxillary right molar extracted. They were divided into three groups of 15. In Group 1 normal saline, Group 2 DOX, and Group 3 EM were administered at the doses of 5 ml/kg/day, 5 mg/kg/day, and 2 mg/kg/day, respectively, for 7 consecutive days. The rats were sacrificed 7, 14, and 21 days after surgery. Real-time polymerase chain reaction was employed to evaluate the mRNA expression of receptor activator of nuclear factor κB ligand (RANKL) and osteoprotegerin (OPG) and immunohistochemical staining for tartrate-resistant acid phosphatase (TRAP) to determine osteoclasts. The data were analyzed by one-way analysis of variance followed by Tukey's
post hoc
test using SPSS version 20. Significant level was set at 0.05.
Results:
The results showed that when drug-treated groups compared to control groups, RANKL gene expression significantly decreased, TRAP
+
cells decreased on day 7. The RANKL/OPG ratios in the first two weeks in the test groups were significantly lower than the control group. There was no significant difference in the studied indices between DOX and EM groups.
Conclusion:
Following administration of DOX and EM, the number of osteoclasts and RANKL/OPG ratio decreased suggesting their anti-osteoclastogenesis activity. These two drugs have no advantage over each other in increasing the bone formation.
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Original Article:
Analysis of lipid profile in cancer patients, smokers, and nonsmokers
A Vikramsimha Reddy, Lakshmi Keerthana Killampalli, A Ravi Prakash, Sushma Naag, G Sreenath, Sunil Kumar Biraggari
Dent Res J
2016, 13:494 (29 December 2016)
DOI
:10.4103/1735-3327.197036
PMID
:28182070
Background:
Lipids play an important role in maintaining the cell membrane integrity. Lipid profile is a panel of blood tests that serve as an initial medical screening for abnormalities in lipids and approximate risk for cancer, cardiovascular diseases, pancreatitis, etc., The present study evaluates the alterations in lipid profile in cancer patients, smokers, and nonsmokers and aims to achieve a correlation between them.
Materials and Methods:
The study is an
in vitro
type of cross-sectional study with 25 oral cancer patients, 25 chronic smokers (habit persisting for 15 years or more), and 15 nonsmokers as control group. Blood samples had been collected, and triglycerides (TGs), total cholesterol (TC), high-density lipoprotein (HDL), very low-density lipoprotein (VLDL), and low-density lipoprotein (LDL) were analyzed using a lipid profile kit and an autoanalyzer. The results were analyzed using the unpaired
t
-test and ANOVA test (
P
< 0.05).
Results:
There was a significant increase in TC, TG, LDL, and VLDL and decrease in HDL in the smokers group when compared to the controls (
P
< 0.05). A significant increase in LDL, but a decrease in values of HDL, VLDL, TG, and TC was observed in the cancer patients group when compared to the controls (
P
< 0.05).
Conclusion:
There is an inverse relationship between serum lipid profile in smokers and cancer patients. The decrease in lipid profile in cancer patients might be due to their increased utilization of lipids by neoplastic cells in membrane biogenesis. Therefore, a decrease in lipid profile in smokers can be assumed that they might be more prone to develop cancerous conditions.
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Original Article:
Design, formulation, and physicochemical evaluation of periodontal propolis mucoadhesive gel
Abolfazl Aslani, Negar Malekpour
Dent Res J
2016, 13:484 (29 December 2016)
DOI
:10.4103/1735-3327.197037
PMID
:28182054
Background:
Periodontitis is a disease of tooth supporting tissues, and Gram-negative
Bacteria
are the main cause of this. Propolis has antibacterial, anti-inflammatory, and antioxidant effects due to its high polyphenol and flavonoids content. The aim of this study is the formulation of a mucoadhesive gel containing concentrated extract of propolis for treatment of periodontitis.
Materials and Methods:
Formulations containing carbopol 940, sodium carboxymethylcellulose (NaCMC), hydroxypropyl methylcellulose K4M, and propolis extract were prepared. Among ten prepared formulations, five formulations had acceptable and proper physical appearance and uniformity; thus, they were selected for physicochemical tests (centrifugal, thermal change, cooling and heating, freeze and thaw, thermal stress, and pH evaluation), quantification of flavonoids, viscosity, mucoadhesion, drug release, and syringeability tests. We investigated the antibacterial activity of F
10
(carbopol 940 1%, NaCMC 3%) against
Porphyromonas gingivalis
using the disk diffusion method.
Results:
Phenolic content was measured 39.02 ± 3.24 mg/g of concentrated extract as gallic acid and flavonoid content was determined 743.28 ± 12.1 mg/g of concentrated extract as quercetin. Highest viscosity (3700 cps) and mucoadhesion (21 MPa) were seen in F
10
. Study of release profile in F
10
also revealed that propolis could release from this system in more than 7 days. All of the five selected formulations had ease of syringeability in 21-gauge needle for drug delivery into periodontal pocket. In the disk diffusion method, F
10
produced significant growth inhibition zones against
P. gingivalis
.
Conclusion:
Controlled release of drug into periodontal pocket helps effective treatment and recovery, higher persistence and reduces drug use frequency. Increase of carbopol 940 leads to viscosity and mucoadhesion elevation and accordingly decreases of release rate.F
10
was the best formulation because of highest viscosity and mucoadhesion and lowest release rate. It had efficient function in treatment of periodontitis, so we recommend it for clinical evaluation.
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Original Article:
Botox as an adjunct to lip repositioning for the management of excessive gingival display in the presence of hypermobility of upper lip and vertical maxillary excess
Lobna Abdel Aziz Aly, Nelly Ibrahim Hammouda
Dent Res J
2016, 13:478 (29 December 2016)
DOI
:10.4103/1735-3327.197039
PMID
:28182056
Background:
Excessive gingival display (GD) is a frequent finding that can occur because of various intraoral or extraoral etiologies. This work describes the use of a mucosal repositioned flap for the management of a gummy smile associated with vertical maxillary excess (VME) and hypermobility of the upper lip followed by injection of Botox.
Materials and Methods:
Seven female patients in the age range of 17–25 years presented with a gummy smile. At full smile, the average GD ranged from 6 to 8 mm. A clinical examination revealed hypermobility of the upper lip. A cephalometric analysis pointed to the presence of VME. The mucosal repositioned flap surgery was conducted followed by injection with botulinum toxin type A (Botox) 2 weeks postsurgically.
Results:
After 4 weeks, results were definitely observed with a decrease from 8 mm gingival exposure to 3 mm, which was considered as normal GD for an adult during smiling.
Conclusion:
For patients desiring a less invasive alternative to orthognathic surgery, the mucosal repositioned flap is a viable alternative. Moreover, Botox is a useful adjunct to enhance the esthetics and improve patient satisfaction where surgery alone may prove inadequately in moderate VME.
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Case Report:
A case report and short review on changing trends in the site of occurrence of adenomatoid odontogenic tumor: Unravelling the past 15 years
Sneha Sethi, Manish Kumar, Pratul Aggarwal, HS Indra Kumar, Chetan D Sugandhi, Silvie Singh
Dent Res J
2016, 13:462 (14 October 2016)
DOI
:10.4103/1735-3327.192312
PMID
:27857774
Adenomatoid odontogenic tumor (AOT) is an uncommon benign odontogenic lesion, with debatable histogenesis and variable histopathology. A systematic and diverse insight into the evolution, clinical presentation, histology, and immunohistochemical findings of this lesion is reviewed and presented. We reviewed the data published from 2000 to 2014 of approximately 255 cases that revealed a significant change in the incidence of predominant site involved, in contrast to the findings published by Reichart. We have also included the chronological order of events leading to the coining of the term AOT, which shows the curiosity that has been dedicated to understanding the lesion. Immunohistochemistry is considered to be a hallmark in pathology for learning the molecular pathogenesis and giving a correct final diagnosis. Several markers have been used to investigate and understand this lesion, and a compilation of the findings has been tabulated.
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Case Report:
A case-report of delayed repositioning of intruded permanent maxillary central incisors accompanied by complicated crown fractures: A 2-year follow-up
Azade Rafiee, Hamid Moradian, Maryam Ayatollahi
Dent Res J
2016, 13:458 (14 October 2016)
DOI
:10.4103/1735-3327.192310
PMID
:27857773
Intrusive luxation is the most severe type of dental injury with a complex healing sequence. Pulp necrosis, root resorption (surface, inflammatory and replacement resorption), and defects in marginal periodontal bone healing are the main complications. Treatment strategies can be either active, by repositioning (surgical or orthodontic extrusion), or passive, by spontaneous re-eruption based on the thorough evaluation of the case. This paper reports a case of delayed repositioning of severely intruded permanent maxillary central incisors accompanied by complicated crown fractures after 3 months. After thorough clinical and radiographic evaluations, and based on guidelines, the teeth were surgically repositioned and splinted for 6 weeks. One week after the initial intervention, the endodontic treatment for both permanent maxillary incisors were initiated using calcium hydroxide. 6 months later, the teeth were ready for MTA plug and gutta-percha root canal filling. During the follow-up period, the teeth had remained functional and esthetically acceptable. Further yearly observations are planned at least for 5 years.
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Case Report:
Stafne bone cavity with ectopic salivary gland tissue in the anterior of mandible
Parviz Deyhimi, Soheila Darisavi, Saeideh Khalesi
Dent Res J
2016, 13:454 (14 October 2016)
DOI
:10.4103/1735-3327.192306
PMID
:27857772
Stafne bone cavities (SBCs) are uncommon well-demarcated defects of the mandible, which often occur in the posterior portion of the jaw bone and are usually asymptomatic. Furthermore, SBC is found in men aged 50-70-year-old. Anterior mandibular variants of SBC are very rare. This article describes a case of anterior SBC in a 45-year-old man that resembled endodontic periapical lesions. Upon histopathological examination, it turned out to be a normal salivary gland tissue.
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Original Article:
A histologic, histomorphometric, and radiographic comparison between two complexes of CenoBoen/CenoMembrane and Bio-Oss/Bio-Gide in lateral ridge augmentation: A clinical trial
Babak Amoian, Ehsan Moudi, Maryam Seyed Majidi, SM Ali Tabatabaei
Dent Res J
2016, 13:446 (14 October 2016)
DOI
:10.4103/1735-3327.192304
PMID
:27857771
Background:
Several grafting materials have been used for alveolar ridge augmentation. The literature lacks researches to compare CenoBone to other grafting materials. The aim of this study was to compare CenoBone/CenoMembrane complex to Bio-Oss/Bio-Gide complex in lateral alveolar bone augmentation in terms of radiographic, histologic, and histomorphometric parameters.
Materials and Methods:
In this randomized controlled trial, ten patients who needed lateral ridge augmentation were selected and augmentations were done using either of CenoBone/CenoMembrane or Bio-Oss/Bio-Gide complexes. In the re-entry surgery in 6 months following augmentation, core biopsies were taken and clinical, radiographic, histologic, and histomorphometric evaluations were performed.
Results:
No statistically significant difference was seen between groups except for the number of blood vessels and percentage of residual graft materials.
Conclusion:
CenoBone seems to present a comparable lateral ridge augmentation to Bio-Oss in.
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Original Article:
Assessment of dimensional accuracy of preadjusted metal injection molding orthodontic brackets
Shiva Alavi, Farnaz Tajmirriahi
Dent Res J
2016, 13:440 (14 October 2016)
DOI
:10.4103/1735-3327.192299
PMID
:27857770
Background:
the aim of this study is to evaluate the dimensional accuracy of McLaughlin, Bennett, and Trevisi (MBT) brackets manufactured by two different companies (American Orthodontics and Ortho Organizers) and determine variations in incorporation of values in relation to tip and torque in these products.
Materials and Methods:
In the present analytical/descriptive study, 64 maxillary right central brackets manufactured by two companies (American Orthodontics and Ortho Organizers) were selected randomly and evaluated for the accuracy of the values in relation to torque and angulation presented by the manufacturers. They were placed in a video measuring machine using special revolvers under them and were positioned in a manner so that the light beams would be directed on the floor of the slot without the slot walls being seen. Then, the software program of the same machine was used to determine the values of each bracket type. The means of measurements were determined for each sample and were analyzed with independent
t
-test and one-sample
t
-test.
Results:
Based on the confidence interval, it can be concluded that at 95% probability, the means of tip angles of maxillary right central brackets of these two brands were 4.1-4.3° and the torque angles were 16.39-16.72°. The tips in these samples were at a range of 3.33-4.98°, and the torque was at a range of 15.22-18.48°.
Conclusion:
In the present study, there were no significant differences in the angulation incorporated into the brackets from the two companies; however, they were significantly different from the ti
P
values for the MBT prescription. In relation to torque, there was a significant difference between the American Orthodontic brackets exhibited significant differences with the reported 17°, too.
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Original Article:
Socket preservation using freeze-dried bone allograft with and without plasma rich in growth factors in dogs
Mohammad Hasan Samandari, Abbas Haghighat, Nakisa Torabinia, Mehdi Taghian, Leyli Sadri, Vahid Naemy
Dent Res J
2016, 13:432 (14 October 2016)
DOI
:10.4103/1735-3327.192290
PMID
:27857769
Background:
Plasma rich in growth factors (PRGF) and freeze-dried bone allograft (FDBA) are shown to promote bone healing. This study was aimed to histologically and histomorphometrically investigate the effect of combined use of PRGF and FDBA on bone formation, and compare it to FDBA alone and control group.
Materials and Methods:
The distal roots of the lower premolars were extracted bilaterally in four female dogs. Sockets were randomly divided into FDBA + PRGF, FDBA, and control groups. Two dogs were sacrificed after 2 weeks and two dogs were sacrificed after 4 weeks. Sockets were assessed histologically and histomorphometrically. Data were analyzed by Kruskal-Wallis test followed by Mann-Whitney U-tests utilizing the SPSS software version 20.
P
< 0.05 was considered statistically significant.
Results:
While the difference in density of fibrous tissue in three groups was not statistically significant (
P
= 0.343), the bone density in grafted groups was significantly higher than the control group (
P
= 0.021). The least decrease in all socket dimensions was observed in the FDBA group. However, these differences were only significant in coronal portion at week 4. Regarding socket dimensions and bone density, the difference between FDBA and FDBA+PRGF groups was not significant in middle and apical portions.
Conclusion:
The superiority of PRGF+FDBA overFDBA in socket preservation cannot be concluded from this experiment.
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Original Article:
Comparative assessment of antimicrobial efficacy of different hand sanitizers: An
in vitro
study
Vardhaman Mulchand Jain, Gundabaktha Nagappa Karibasappa, Arun Suresh Dodamani, Vishwakarma K Prashanth, Gaurao Vasant Mali
Dent Res J
2016, 13:424 (14 October 2016)
DOI
:10.4103/1735-3327.192283
PMID
:27857768
Background:
To evaluate the antimicrobial efficacy of four different hand sanitizers against
Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa
,
Escherichia coli
, and
Enterococcus faecalis
as well as to assess and compare the antimicrobial effectiveness among four different hand sanitizers.
Materials and Methods:
The present study is an
in vitro
study to evaluate antimicrobial efficacy of Dettol, Lifebuoy, PureHands, and Sterillium hand sanitizers against clinical isolates of the aforementioned test organisms. The well variant of agar disk diffusion test using Mueller-Hinton agar was used for evaluating the antimicrobial efficacy of hand sanitizers. McFarland 0.5 turbidity standard was taken as reference to adjust the turbidity of bacterial suspensions. Fifty microliters of the hand sanitizer was introduced into each of the 4 wells while the 5
th
well incorporated with sterile water served as a control. This was done for all the test organisms and plates were incubated in an incubator for 24 h at 37C. After incubation, antimicrobial effectiveness was determined using digital caliper (mm) by measuring the zone of inhibition.
Results:
The mean diameters of zones of inhibition (in mm) observed in Group A (Sterillium), Group B (PureHands), Group C (Lifebuoy), and Group D (Dettol) were 22 ± 6, 7.5 ± 0.5, 9.5 ± 1.5, and 8 ± 1, respectively. Maximum inhibition was found with Group A against all the tested organisms. Data were statistically analyzed using analysis of variance, followed by
post hoc
test for group-wise comparisons. The difference in the values of different sanitizers was statistically significant at
P
< 0.001.
Conclusion:
Sterillium was the most effective hand sanitizer to maintain the hand hygiene.
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Original Article:
Exploring the missing link - Empathy among dental students: An institutional cross-sectional survey
Vikram Pal Aggarwal, Robin Garg, Nikita Goyal, Puneet Kaur, Sakshi Singhal, Nancy Singla, Deeksha Gijwani, Aditi Sharma
Dent Res J
2016, 13:419 (14 October 2016)
DOI
:10.4103/1735-3327.192279
PMID
:27857767
Background:
Empathy plays an important role in healthy dentist and patient relationship. Hence, the aim of the study is to (a) to measure the self-reported empathy levels among dental undergraduate and postgraduate students. (b) To review the trend of changes in empathy level with experience, age, and gender among dental undergraduate and postgraduate students.
Materials and Methods:
This cross-sectional, questionnaire-based study was carried out in two private dental institutions situated in Sri Ganganagar, India, with a sample size of 978. Data were obtained from the 1
st
to final year (BDS), interns, and postgraduate students from January to March 2015. An empathy level of students was assessed by the Jefferson Scale of Physician Empathy - Health Profession Students Version Questionnaire. An exploratory factor analysis using Kaiser's criteria was undertaken to appraise the construct validity and dimensionality. Based on the results of the factor analysis, three factors were selected; labeled as "perspective taking," "compassionate care," and "standing in patient's shoes."
Results:
The majority of the students was female in a equivalent ratio of 1338:618. There were significant differences in empathy scores by gender and age (
P
< 0.01). The lowest and highest mean empathy scores were found in postgraduate (mean = 108.77, standard deviation [SD] =9.12) and 1
st
year (mean = 117.23, SD = 14.19) dental students, respectively.
Conclusion:
Dental educators should consider the likely decline in empathy among students as early as possible and adopt communication teaching strategies to promote the development of empathy and reduce the risk of further decline.
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Original Article:
Serum level of Interleukin-8 in subjects with diabetes, diabetes plus oral lichen planus, and oral lichen planus: A biochemical study
Atefeh Tavangar, Faezeh Khozeimeh, Fereshtehossadat Ghoreishian, Mahsa Abbasi Boroujeni
Dent Res J
2016, 13:413 (14 October 2016)
DOI
:10.4103/1735-3327.192277
PMID
:27857766
Background:
Correlation between diabetes mellitus (DM) and oral lichen planus (OLP) seems probable. Since Interleukin-8 (IL-8) is an important inflammatory mediator involved in both conditions, this study aimed to measure and compare the serum level of IL-8 in DM, OLP, and DM + OLP patients in comparison with healthy individuals.
Materials and Methods:
This cross sectional study was conducted on 75 patients (30 OLP, 5 OLP and type II DM, 20 type II DM, and 20 healthy controls). Serum levels of IL-8, fasting blood sugar (FBS) and 2-h postprandial blood sugar were measured in the four groups. Data were analyzed using SPSS version 20 by one-way ANOVA and post_hocleast significant difference test.
Results:
Type II DM patients with OLP had the highest mean serum level of IL-8 followed by OLP, DM and control groups, respectively. Pairwise comparison of groups revealed significant differences in serum IL-8 between the control and OLP and also control and OLP+DM (
P
< 0.05) groups. No other significant differences were noted. The mean levels of FBS and 2-h postprandial blood sugar were the highest in OLP+DM patients followed by DM, OLP and control groups, respectively.
Conclusion:
The ascending trend of serum level of IL-8 in the control, DM, OLP, and DM+OLP patients may indicate the role of this factor in the pathogenesis of DM and OLP. Moreover, it may play a synergistic role in patients suffering from both conditions.
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Original Article:
Effect of Cyclosporin A and Angiotensin II on cytosolic calcium levels in primary human gingival fibroblasts
Ajitkumar Supraja, Murugan Girija Dinesh, Subbarayan Rajasekaran, Thodur Madapusi Balaji, Suresh Ranga Rao
Dent Res J
2016, 13:405 (14 October 2016)
DOI
:10.4103/1735-3327.192276
PMID
:27857765
Background:
To evaluate the effect of Cyclosporin A (CsA) and angiotensin II (Ang II) on cytosolic calcium levels in cultured human gingival fibroblasts (HGFs).
Materials and Methods:
Healthy gingival samples from six volunteers were obtained, and primary HGFs were cultured. Cell viability and proliferation assay were performed to identify the ideal concentrations of CsA and Ang II. Cytosolic calcium levels in cultured gingival fibroblasts treated with CsA and Ang II were studied using colorimetric assay, confocal and fluorescence imaging. Statistical analyses were done using SPSS software and GraphPad Prism.
Results:
Higher levels of cytosolic levels were evident in cells treated with CsA and Ang II when compared to control group and was statistically significant (
P
< 0.05) in both colorimetric assay and confocal imaging. Fluorescent images of the cultured HGFs revealed the same.
Conclusion:
Thus calcium being a key player in major cellular functions, plays a major role in the pathogenesis of drug-induced gingival overgrowth.
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Original Article:
Dimensional and volumetric analysis of the oropharyngeal region in obstructive sleep apnea patients: A cone beam computed tomography study
Tripti Tikku, Rohit Khanna, Kiran Sachan, Akhil Agarwal, Kamna Srivastava, Anubha Lal
Dent Res J
2016, 13:396 (14 October 2016)
DOI
:10.4103/1735-3327.192273
PMID
:27857764
Background:
Obstructive Sleep Apnea (OSA) is a potentially life-threatening condition in which there is a periodic cessation of breathing (for 10 sec or longer) that occurs during sleep in the presence of inspiratory effort. The aim of the study was to assess volumetric and dimensional differences between OSA patients and normal individuals in the upright posture.
Material and Method:
The present study was conducted on CBCT scans of 32 patients who were divided into two groups -Group I (control group) and Group II (OSA subjects). Group I consisted of 16 patients with normal airway with ESS score from 2 to 10, STOP BANG Questionnaire score of <3 and who had undergone CBCT for various diagnostic reasons. Group II had patients with ESS score >10, STOP BANG Questionnaire score of > 3, AHI index >5. Linear and angular parameters, volume and minimum cross-section area (MCA) of oropharyngeal airway, anteroposterior length and lateral width at MCA was compared amongst the groups.
Results:
The oropharyngeal volume, MCA, and the anteroposterior and lateral width of the airway at MCA of the OSA subjects was significantly lesser than that of normal subjects. The length of both soft palate and tongue was significantly more in Group II. The angle between the nasopharyngeal airway and the oropharyngeal airway was significantly more obtuse in Group II.
Conclusion:
The reduction in oropharyngeal volume in OSA patients could be attributed to different anatomical and pathophysiological factors that were corroborated with the findings of the present study.
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Review Article:
Prevalence of malocclusion among Iranian children: A systematic review and meta-analysis
Maryam Akbari, Kamran Bagheri Lankarani, Behnam Honarvar, Reza Tabrizi, Hossein Mirhadi, Mahmood Moosazadeh
Dent Res J
2016, 13:387 (14 October 2016)
DOI
:10.4103/1735-3327.192269
PMID
:27857763
Background:
The high prevalence of malocclusion is a public health problem in the world and the third priority in oral care. Numerous primary studies have presented reports on the prevalence of malocclusion among Iranian children. In combination, the results of these studies using meta-analysis are highly valuable for health policy-making. Similarly, this study aimed at determining the prevalence of different types of malocclusion among Iranian children.
Materials and Methods:
Using relevant keywords, national and international databases were explored. After narrowing down the search strategy and leaving out the duplicates, the remaining articles were screened based on titles and abstracts. To increase search sensitivity, reference lists of the papers were examined. To identify unpublished articles and documentations, a set of negotiations were done with the people involved and research centers. Finally, the heterogeneity index between the studies was determined using Cochran (Q) and
I
2
tests. According to the results of heterogeneity, the random effects model was used to estimate the prevalence of malocclusion in Iran.
Results:
In total, 25 articles were included in the meta-analysis process. The prevalence of dental malocclusion was estimated in 28,693 Iranian children aged 3-18 years. The total prevalence of Class I, II, and III malocclusion was 54.6% (46.5-62.7), 24.7% (20.8-28.7), and 6.01% (4-7.1), respectively. The prevalence of Class I, II, and III malocclusion was 44.6% (32.9-56.2), 21.5% (18.01-25.1), and 4.5% (3.2-5.9) in boys and 48.8% (36.8-60.8), 21.5% (16.9-25.1), and 5.5% (3.9-7.1) in girls, respectively.
Conclusion:
This study showed a high prevalence of malocclusion among Iranian children. Also, the results indicated that the prevalence is higher in girls.
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Review Article:
Scaffolds in regenerative endodontics: A review
Kinjal M Gathani, Srinidhi Surya Raghavendra
Dent Res J
2016, 13:379 (14 October 2016)
DOI
:10.4103/1735-3327.192266
PMID
:27857762
Root canal therapy has enabled us to save numerous teeth over the years. The most desired outcome of endodontic treatment would be when diseased or nonvital pulp is replaced with healthy pulp tissue that would revitalize the teeth through regenerative endodontics. 'A search was conducted using the Pubmed and MEDLINE databases for articles with the criteria 'Platelet rich plasma', 'Platelet rich fibrin', 'Stem cells', 'Natural and artificial scaffolds' from 1982-2015'. Tissues are organized as three-dimensional structures, and appropriate scaffolding is necessary to provide a spatially correct position of cell location and regulate differentiation, proliferation, or metabolism of the stem cells. Extracellular matrix molecules control the differentiation of stem cells, and an appropriate scaffold might selectively bind and localize cells, contain growth factors, and undergo biodegradation over time. Different scaffolds facilitate the regeneration of different tissues. To ensure a successful regenerative procedure, it is essential to have a thorough and precise knowledge about the suitable scaffold for the required tissue. This article gives a review on the different scaffolds providing an insight into the new developmental approaches on the horizon.
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Case Report:
Maxillary tooth displacement in the infratemporal fossa
Korosh Roshanghias, Andre Peisker, Jorg Olaf Zieron
Dent Res J
2016, 13:373 (5 August 2016)
DOI
:10.4103/1735-3327.187881
PMID
:27605997
Wisdom tooth operations are sometimes accompanied by complications. This case report shows complications during upper jaw third molar removal. Expectable problems during oral surgery should be planned to be solved in advance. Displacement of the third molar during oral surgeries as a considerable complication is rarely discussed scientifically. A good design of flap, adequate power for extraction, and clear view on the surgical field are crucial. Three-dimensional radiographic diagnostics in terms of cone beam computed tomography is helpful after tooth displacement into the infratemporal fossa.
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Original Article:
The effect of intrusive orthodontic force on dental pulp of adults versus adolescents
Mostafa Abtahi, Neda Eslami, Reza Zare Mahmoud Abadi, Saber Pooria Rezaei
Dent Res J
2016, 13:367 (5 August 2016)
DOI
:10.4103/1735-3327.187877
PMID
:27605996
Background:
There is no conclusive evidence on the effect of orthodontic force application on dental pulp tissue. The aim of this study was to compare early and delayed histological effects of intrusive forces on dental pulp of adolescents and adults.
Materials and Methods:
Patients referred to the Department of Orthodontics of Mashhad University of Medical Sciences participated in this study. They were assigned to adult (25–32-year-old) and adolescent (13–18-year-old) Groups. A cantilever spring made of 16 × 22 steel wire was used to apply intrusive force to upper first premolars (11 teeth in adolescents and 11 teeth in adults) and the opposing teeth were considered as control group. In each group, 6 pairs of teeth were extracted after one week, and the remaining 5 pairs were extracted after one month of intrusion. Histologic changes were compared between the control and intrusive groups and also between the adults and adolescents after 7 days and 1 month. Statistical analysis was performed using Statistical Package for the Social Sciences and Wilcoxon and Mann–Whitney U-tests.
P
≤ 0.05 was set as statistically significant.
Results:
Significant difference was not found in any histological parameters between intrusive and control groups 1 week and 1 month after intrusion in adolescents and adults (
P
> 0.05). One month after intrusion, inflammatory cell response intensity (
P
= 0.032) and frequency of chronic inflammation (
P
= 0.032) were significantly higher in adults compared to adolescents.
Conclusion:
Mild intrusive force in closed apex teeth causes no significant histologic changes in adolescents and adults. However, it seems that inflammatory-related histologic pulpal changes are more severe in adults after one month of intrusion.
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Original Article:
Radicular anatomy of permanent mandibular second molars in an Iranian population: A preliminary study
Nahid M Akhlaghi, Fatemeh Mashadi Abbas, Mostafa Mohammadi, Mohammad Reza Karami Shamloo, Orkideh Radmehr, Ramin Kaviani, Vahid Rakhshan
Dent Res J
2016, 13:362 (5 August 2016)
DOI
:10.4103/1735-3327.187883
PMID
:27605995
Background:
Root morphology is of utmost importance to endodontic sciences. Since there are a few studies on the morphology of mandibular second molars' roots, and some anatomical variables are not evaluated before, the aim of this study was to investigate thoroughly radicular anatomy of this tooth.
Materials and Methods:
This
ex vivo
study was performed on 150 intact mandibular second molars. After access cavity preparation and ensuring canal patency, Indian ink was injected into root canals from the orifices. The teeth became transparent using methyl salicylate storage. Then, they were inspected by an endodontist under a ×10 stereomicroscope regarding numerous root morphological variables. Data were analyzed using chi-square test and analysis of variance (α = 0.05).
Results:
About 86.7% of teeth had two roots and 13.3% were single-rooted (
P
= 0.0001), of which, 50% were C-shaped (6.7% of all teeth,
P
= 0.0001). 86.7% of mesial roots were double canalled, whereas 75.3% of distal roots were single canalled (
P
= 0.0001). 71.45% and 95.3% of the mesial and distal roots had one apical foramen, respectively (
P
= 0.0001). Apical foramens were mostly central followed by lingual in most cases. Distances between apical foramen and apical constriction ranged between 0.27 and 0.40 mm (
P
= 0.0545). Distances between apical foramen and root apices ranged between 0.30 and 0.47 mm (
P
= 0.0001). Vertucci classifications of mesial canals were Type II in 62.6% and Type IV in 37.4%. 86.2% of single-canal distal roots were Type I. 66.7% of double-canal distal roots were Type II and 33.3% were Type IV (
P
= 0.0001). The mean root lengths from cervical to apex of mesial, distal, and single roots were 14.02 ± 0.85 (95% confidence interval [CI] = 13.87–14.17), 13.35 ± 0.91 (95% CI = 13.19–13.50), and 14.25 ± 0.72 mm (95% CI = 13.91–14.58), respectively. The extents of canal curvatures varied between 20° and 31° buccolingually (
P
= 0.0000), and between 19° and 27° mesiodistally (
P
= 0.0000).
Conclusion:
There was a considerable rate of eccentric apical foramen in mandibular second molars.
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Original Article:
Correlation of interleukin-6-174 GC and interleukin-6-572 GC gene polymorphisms with periodontal disease in an Iranian population
Bahareh Nazemi Salman, Surena Vahabi, Alireza Biglari, Simindokht Salavitabar, Maryam Hassani Doabsari
Dent Res J
2016, 13:354 (5 August 2016)
DOI
:10.4103/1735-3327.187884
PMID
:27605994
Background:
Periodontal disease has a multifactorial etiology. A combination of microbial agents and environmental, habitual, systemic, and genetic risk factors is responsible for the development of periodontal disease. Host immune response causes the destruction of tooth-supporting structure and eventual tooth loss. This study aimed to assess the correlation of interleukin 6 (IL-6) -174-GC and IL-6-572-GC gene polymorphisms with periodontal disease in an Iranian population.
Materials and Methods:
This case–control analytical study was conducted on 129 subjects presenting to the laboratory of Taleghani Hospital. Subjects underwent clinical and periodontal examinations and divided into five groups of healthy, gingivitis and mild, moderate and severe periodontitis. Blood samples (2 ml) were obtained. Genomic DNA was extracted manually using the salting-out method. IL-6 sequence amplification was performed using polymerase chain reaction with three thermal protocols. Digested products were analyzed by electrophoresis through 2% agarose gel using Gel Red staining. Data were analyzed using Chi-square, Kruskal–Wallis, and Mann–Whitney tests, and
P
< 0.05 was considered significant.
Results:
The frequency of GG polymorphism at IL-6-174 and IL-6-572 genomic regions was 51.2% and 71.3%, respectively. The frequency of IL-6-572-GG polymorphism was significantly greater than that of IL-6-572-GC polymorphism (
P
< 0.001). Comparison of the mean and maximum pocket depth and clinical attachment level, as well as bleeding on probing percentage, revealed significant differences between the healthy controls and periodontitis patients (
P
< 0.001). The frequency percentages of GC and GG polymorphisms were almost equal in the healthy, gingivitis, and periodontitis groups. In other words, the frequency of the two polymorphisms was not significantly different between the health and disease states (
P
= 0.065 for IL-6-572 and
P
= 0.63 for IL-6-174).
Conclusion:
This study found no association between IL-6-174 and IL-6-572 gene polymorphisms and periodontitis in the studied population.
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Original Article:
Oral health knowledge, behaviour and practices among school children in Qatar
Mohammed Sultan Al-Darwish
Dent Res J
2016, 13:342 (5 August 2016)
DOI
:10.4103/1735-3327.187885
PMID
:27605993
Background:
The aim of this study was to assess the oral health knowledge behaviour and practices among school children in Qatar.
Materials and Methods:
A cross-sectional study was carried out in Qatar from October 2011 to April 2012. A total of 2200 school children aged 12–14 years were approached from 16 schools of different areas. The information about oral health knowledge and sources of information was obtained through a self-administrated questionnaire. Data analyses were performed.
Results:
The overall response rate was (96%). Only (25.8%) of children reported a high level of oral health knowledge. After each meal, tooth brushing was observed by a very low percentage of children (3.7%). About 44.6% of children recognized dental floss as a cleaning device for between the teeth. A large number of children (32.5%) thought incorrectly that one must visit the dentist only in case of pain. A great majority was not aware of cariogenic potential of soft drinks (39%) and sweetened milk (97.8%). Less than half (38.9%) of children actually had heard about fluoride. Only (16.8%) correctly answered the question about sign of tooth decay. Slightly, less than half (48.4%) could not define the meaning of plaque. Parents were the most popular (69.1%), source of oral health information for the children.
Conclusion:
The oral health knowledge in Qatar is below the satisfactory level. Parents were the most popular source of oral health knowledge for the children followed by dentists, school teachers, and media.
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Original Article:
Evaluation of salivary nitric oxide level in children with early childhood caries
AR Senthil Eagappan, V Arun Prasad Rao, S Sujatha, D Senthil, J Sathiyajeeva, G Rajaraman
Dent Res J
2016, 13:338 (5 August 2016)
DOI
:10.4103/1735-3327.187882
PMID
:27605992
Background:
Nitric oxide (NO), a highly reactive radical, participates in the nonspecific natural defense mechanism of the oral cavity. The present study was attempted to evaluate the salivary NO levels in 4–5 year-old children with early childhood caries (ECC). The objective of the present study was to assess the salivary NO concentration in children with different caries activity.
Materials and Methods:
The study included 120 healthy 4–5 year-old children and they were equally divided into three groups based on decayed, missing, filled surfaces (dmfs) score; forty caries-free children (control group), forty children with dmfs 1–5 (ECC group), and forty with dmfs ≥6 (severe ECC group). Saliva collected was measured for NO concentration by Griess reaction method. The obtained data were analyzed by ANOVA and Pearson's correlation coefficient.
Results:
The mean level of NO in the saliva of the control group was 51.2 ± 8.3457 and that of ECC and severe ECC were 47.1 ± 5.2614 and 33.625 ± 4.6942, respectively. The mean salivary NO concentration was significantly higher in healthy controls when compared to children with ECC and severe ECC. Moreover, a negative correlation (
r
= −0.6658) was observed between the salivary NO level and the mean dmfs, suggesting that as the salivary NO level decreases, the caries incidence increases.
Conclusion:
The obtained results support the antimicrobial activity of salivary NO and also suggest that an increase in NO production might contribute to lower the caries occurrence in children.
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Original Article:
A survey on the effects of metabolic syndrome on the periodontal indices of hemodialysis patients
Mohammad Tavakoli, Mozhgan Izadi, Jaber Yaghini, Abdolah Rastegari, Ahmad Moghareh Abed
Dent Res J
2016, 13:333 (5 August 2016)
DOI
:10.4103/1735-3327.187873
PMID
:27605991
Background:
The metabolic syndrome and periodontitis coincide with systemic inflammation and glucose tolerance disorder, which indicate the common pathophysiologic pathway of these diseases. The main goal of this study was to determine the effects of the metabolic syndrome on the periodontal indices of hemodialysis (HD) patient.
Materials and Methods:
In this descriptive-analytical study, 75 persons were selected by the simple method, 50 of them were HD patients, and 25 of them were healthy people. They were divided into three groups each of which included 25 persons. The periodontal conditions of the selected persons were determined by radiography, gingival index, probing pocket depth, and bleeding index. Then, the periodontal indices of the patients suffering from metabolic syndrome and the persons not affected by metabolic syndrome were compared. At the end, the data were analyzed by SPSS software (version 20) using Mann–Whitney and Kruskal–Wallis tests at the meaningful level of α = 0.05. Since the condition of the unilateral variance test could not be provided for this study, the Kruskal–Wallis test was used. To complete the test, the Mann–Whitney test was used in the binary form among the groups being studied.
Results:
The obtained results showed a meaningful difference among the groups under study (
P
< 0.001). The results of the test showed a meaningful difference among the obtained indices of the groups being studied in the binary form (
P
< 0.001) too.
Conclusion:
The results of the present study revealed when the severity of chronic systemic disease increased the health of the periodontal tissues would decrease.
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Original Article:
Diagnostic value of DIAGNOdent in detecting caries under composite restorations of primary molars
Ava Vali Sichani, Shahrzad Javadinejad, Roshanak Ghafari
Dent Res J
2016, 13:327 (5 August 2016)
DOI
:10.4103/1735-3327.187874
PMID
:27605990
Background:
Direct observation cannot detect caries under restorations; therefore, the aim of this study was to compare the accuracy of radiographs and DIAGNOdent in detecting caries under restorations in primary teeth using histologic evaluation.
Materials and Methods:
A total of 74 previously extracted primary molars (37 with occlusal caries and 37 without caries) were used. Class 1 cavity preparations were made on each tooth by a single clinician and then the preparations were filled with composite resin. The accuracy of radiographs and DIAGNOdent in detecting caries was compared using histologic evaluation. The data were analyzed by SPSS version 21 using Chi-square, Mc Namara statistical tests and receiver operating characteristic curve. The significance was set at 0.05.
Results:
The sensitivity and specificity for DIAGNOdent were 70.97 and 83.72, respectively. Few false negative results were observed, and the positive predictive value was high (+PV = 75.9) and the area under curve was more than 0.70 therefore making DIAGNOdenta great method for detecting caries (
P
= 0.0001). Two observers evaluated the radiographs and both observers had low sensitivity ( first observer: 48.39) (second observer: 51.61) and high specificity (both observers: 79.07). The +PV was lower than DIAGNOdent and the area under curve for both observers was less than 0.70. However, the difference between the two methods was not significant.
Conclusion:
DIAGNOdent showed a greater accuracy in detecting secondary caries under primary molar restorations, compared to radiographs. Although DIAGNOdent is an effective method for detecting caries under composite restorations, it is better to be used as an adjunctive method alongside other detecting procedures.
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Original Article:
Frequency and pattern of idiopathic osteosclerosis and condensing osteitis lesions in panoramic radiography of Iranian patients
Farrokh Farhadi, Mohammad Razavi Ruhani, Ali Zarandi
Dent Res J
2016, 13:322 (5 August 2016)
DOI
:10.4103/1735-3327.187880
PMID
:27605989
Background:
The purpose of this study was to investigate of radiographic pattern and relative frequency of idiopathic osteosclerosis (IO) and condensing osteitis (CO) in panoramic radiographs.
Materials and Methods:
Totally 411 panoramic radiographies were randomly selected from patients referred to Radiology Department of Faculty of Dentistry, Tabriz University of Medical Sciences. Descriptive characteristics of radiopacities, including shape, border, localization, and dental relationship, were recorded. The Chi-squared test was used.
Results:
IO was detected in 31 (7.5%) patients and 22 (68.8%) lesions had exact border while their shape was mostly irregular. About 17 (53.1%) lesions were apical, and 13 (40.6%) lesions had no relation to the teeth. The most involved teeth were the second premolar (28.1%) and first molar (25%). Moreover, CO was detected in 32 (7.8%) patients. 17 (53.1%) lesions had an ill-defined border, and their shape was mostly irregular (65.6%) with ill-defined border. Around 18 (56.3%) lesions were apical, and 11 (34.4) lesions were apical and interradicular. The most involved teeth were the second premolar (59.4%) and the first molar (21.9%).
Conclusion:
The results demonstrated that relative frequency of IO in the selected population was 7.5% and for CO, it was 7.8%.
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Original Article:
Prevalence and patterns of palatine and adenoid tonsilloliths in cone-beam computed tomography images of an Iranian population
Zahra Dalili Kajan, Ali Khalighi Sigaroudi, Majedeh Mohebbi
Dent Res J
2016, 13:315 (5 August 2016)
DOI
:10.4103/1735-3327.187871
PMID
:27605988
Background:
Tonsilloliths are calcified concretions that develop in tonsillar crypts. They are usually small and asymptomatic, so they are found accidentally during routine dental radiogrphy procedure. Large tonsilloliths can occur with clinical signs and symptoms. The purpose of this study was to evaluate the prevalence and patterns of palatine and adenoid tonsilloliths in cone-beam computed tomography (CBCT) images.
Materials and Methods:
In this cross-sectional study, 0.5-mm axial and coronal slices of 134 CBCT images were evaluated to determine the presence of palatine and adenoid calcifications. Their patterns such as being unilateral or bilateral as well as single or multiple and their largest linear sizes were reported.
Results:
Fifty-four (40.3%) patients with palatine tonsilloliths and 17 (12.7%) with adenoid calcifications were found. Thirty (55.6%) palatine tonsilloliths were unilateral, 19 (35.2%) were detected in the left tonsils. Approximately, 54 cases of 78 palatine calcifications were multiple. Seventeen patients had adenoid calcifications that 41.1% of them were unilateral. Fourteen adenoid calcifications were single. The mean ages of patients with palatine tonsilloliths and adenoid calcifications were 45.59 years and 46.53 years, respectively. The range of linear measurements of palatine tonsil calcifications was 0.9–4.2 mm (2.47-mm mean size) while adenoid calcifications ranged from 0.5 to 2.2 mm (0.95-mm mean size). The level of statistical significant difference was <0.05.
Conclusion:
Gender did not affect total prevalence, the pattern of tonsilar calcifications and their linear sizes. The prevalence of tonsilloliths increased with aging, but this variable did not have an effect on their linear size.
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Original Article:
The effect of buccal corticotomy on accelerating orthodontic tooth movement of maxillary canine
Mohammad Reza Jahanbakhshi, Ali Mohammad Kalantar Motamedi, Masoud Feizbakhsh, Ahmad Mogharehabed
Dent Res J
2016, 13:303 (5 August 2016)
DOI
:10.4103/1735-3327.187875
PMID
:27605986
Background:
Selective alveolar corticotomy is defined as an intentional injury to cortical bone. This technique is an effective means of accelerating orthodontic tooth movement. The aim of this study is to evaluate the effect of buccal corticotomy in accelerating maxillary canine retraction.
Materials and Methods:
The sample in this clinical trial study consisted of 15 adult female patients with therapeutic need for extraction of maxillary first premolars and maximum canine retraction. By use of split-mouth design, at the time of premolars extraction, buccal corticotomy was performed around the maxillary first premolar, randomly on one side of maxilla, and the other side was reserved as the control side. Canine retraction was performed by use of friction – less mechanic with simple vertical loop. Every 2 weeks, distance between canines and second premolars was measured until complete space closure. The velocity of space closure was calculated to evaluate the effect of this technique in accelerating orthodontic tooth movement. The obtained data were statistically analyzed using independent
t
-test, and the significance was set at 0.05.
Results:
The rate of canine retraction was significantly higher on the corticotomy side than the control side by an average of 1.8 mm/month versus 1.1 mm/month in the corticotomy side and control side, respectively (
P
< 0.001).
Conclusion:
Based on result of this study, corticotomy can accelerates the rate of orthodontic tooth movement about two times faster than conventional orthodontics and it is significant in early stages after surgical porsedure. Therefore Buccal corticotomy is a useful adjunct technique for accelerating orthodontic tooth movement.
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Case Report:
Definitive guiding flange prosthesis: A definitive approach in segmental mandibulectomy defect
Sumanth Babu, Shaurya Manjunath, Mayuri Vajawat
Dent Res J
2016, 13:292 (10 May 2016)
DOI
:10.4103/1735-3327.182192
PMID
:27274352
Mandibular discontinuity defects following a segmental mandibulectomy defects present a major challenge to the rehabilitation team. With no immediate intervention to rehabilitate the patient, definitive mandibular guidance prostheses with a metal guiding flange and acrylic teeth on the resected side can be used successfully to stabilize the occlusion and correct the deviation. The present case report describes the prosthodontic rehabilitation of a patient with a segmental mandibulectomy using a mandibular prosthesis with a metal guide flange and a maxillary stabilizing metal framework.
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Original Article:
Clinical and radiographic assessment of mineral trioxide aggregate and calcium hydroxide as apexification agents in traumatized young permanent anterior teeth: A comparative study
SG Damle, Hiteshwar Bhattal, Dhanashree Damle, Abhishek Dhindsa, Ashish Loomba, Sumit Singla
Dent Res J
2016, 13:284 (10 May 2016)
DOI
:10.4103/1735-3327.182191
PMID
:27274351
Background:
To evaluate and compare the efficacy of mineral trioxide aggregate (MTA) and traditionally used calcium hydroxide (Ca(OH)
2
) in inducing root end formation of immature roots of traumatized young permanent anterior teeth.
Materials and Methods:
The study was carried out on 22 nonvital, immature permanent maxillary incisors. Samples were allotted into two groups - Group I MTA and Group II Ca(OH)
2
Success rate was determined based upon the time duration required for apical barrier formation. The canals were obturated using gutta-percha points in MTA group, after 24 h, whereas in Ca(OH)
2
group, obturation was carried out after radiographic confirmation of the apical barrier. The clinical and radiographic evaluation was carried out at a follow-up periods of 3, 6, and 9 months and statistical analysis was carried out by SPSS version 15.0 statistical analysis software (Chi-square test and fisher exact test).
Results:
In MTA Group, barrier formation was observed in 90.90% of the patients after 9 months whereas in Ca(OH)
2
Group, the same was observed in 81.81%. The mean time required for barrier formation in MTA group was 4.90 months and 5.33 months in Ca(OH)
2
group.
Conclusion:
MTA and Ca(OH)
2
, as medicaments for apexification, were comparable in terms of the evaluation parameters. However, MTA was beneficial in terms of immediate obturation of immature roots with wide open apices.
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Original Article:
Comparison of patients' age receiving therapeutic services in a cleft care team in Isfahan
Saeed Soheilipour, Fatemeh Soheilipour, Fatemeh Derakhshandeh, Hedieh Hashemi, Mehrdad Memarzadeh, Hamid Salehiniya, Fahimeh Soheilipour
Dent Res J
2016, 13:278 (10 May 2016)
DOI
:10.4103/1735-3327.182190
PMID
:27274350
Background:
Due to numerous difficulties in patients suffering from varieties of cleft lip and palate, their therapeutic management involves interdisciplinary teamwork. This study was conducted to compare the age of commencing treatments such as speech therapy, secondary palate and alveolar bone grafting and orthodontics between those who sought treatment early and late.
Materials and Methods:
In this retrospective study, 260 files of patients with cleft lip and palate based on their age at the time of admission to a cleft care team were divided into two groups: The early admission and late admission. Both groups compared based on four variables including the mean age of beginning speech therapy, palatal secondary surgery, alveolar bone grafting, and receiving orthodontics using
t
-test.
Results
: Based on the results, among 134 patients admitted for speech therapy, the mean age of initiating speech therapy in early clients was 3.3 years, and in the late ones was 9 years. Among 47 patients with secondary surgery, the mean age in early clients was 3.88 years, and in the late clients was 15.7 years. Among 17 patients with alveolar bone grafting, the mean age in the first group was 9 years, and in the other was 16.69 years. Among 24 patients receiving orthodontic services, the mean age in early clients was 7.66 years, and in the second group was 17.05 years.
Conclusion:
There was a significant difference between the age of performing secondary surgery and alveolar bone grafting and the age of beginning speech therapy and receiving orthodontic services in early references and late references to the team.
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Original Article:
Children's behavioral pain reactions during local anesthetic injection using cotton-roll vibration method compared with routine topical anesthesia: A randomized controlled trial
Ali Bagherian, Mahmood Sheikhfathollahi
Dent Res J
2016, 13:272 (10 May 2016)
DOI
:10.4103/1735-3327.182189
PMID
:27274349
Background:
Topical anesthesia has been widely advocated as an important component of atraumatic administration of intraoral local anesthesia. The aim of this study was to use direct observation of children's behavioral pain reactions during local anesthetic injection using cotton-roll vibration method compared with routine topical anesthesia.
Materials and Methods:
Forty-eight children participated in this randomized controlled clinical trial. They received two separate inferior alveolar nerve block or primary maxillary molar infiltration injections on contralateral sides of the jaws by both cotton-roll vibration (a combination of topical anesthesia gel, cotton roll, and vibration for physical distraction) and control (routine topical anesthesia) methods. Behavioral pain reactions of children were measured according to the author-developed face, head, foot, hand, trunk, and cry (FHFHTC) scale, resulting in total scores between 0 and 18.
Results:
The total scores on the FHFHTC scale ranged between 0-5 and 0-10 in the cotton-roll vibration and control methods, respectively. The mean ± standard deviation values of total scores on FHFHTC scale were lower in the cotton-roll vibration method (1.21 ± 1.38) than in control method (2.44 ± 2.18), and this was statistically significant (
P
< 0.001).
Conclusion:
It may be concluded that the cotton-roll vibration method can be more helpful than the routine topical anesthesia in reducing behavioral pain reactions in children during local anesthesia administration.
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Original Article:
Effect of cyclic loading on microleakage of silorane based composite compared with low shrinkage methacrylate-based composites
Hamid Kermanshah, Esmail Yasini, Razieh Hoseinifar
Dent Res J
2016, 13:264 (10 May 2016)
DOI
:10.4103/1735-3327.182188
PMID
:27274348
Background:
There are many concerns regarding the marginal seal of composite restorations, especially when composite restorations are subjected to cyclic loading. The aim of this study was to evaluate the effect of cyclic loading on the microleakage of silorane based composite compared with low shrinkage methacrylate-based composites in class V cavities.
Materials and Methods:
In this
in vitro
study, class V cavities were prepared on the facial and lingual surfaces of 48 human premolars (96 cavities). The teeth were randomly divided into four groups of 12 teeth (24 cavities) each and restored as follows: Group 1 (Siloran System Adhesive + Filtek P90), Group 2 (All Bond SE + Aelite LS Posterior), Group 3 (Futurabond NR + Grandio), and Group 4 (G-Bond + Kalore-GC). All the specimens were thermocycled for 2000 cycles (5-55°C) and then half of the specimens from each group, were Load cycled. All teeth were immersed in 0.5% basic fuchsine dye, sectioned, and observed under a stereomicroscope. Data were analyzed using Wilcoxon test, Kruskal-Wallis, and Mann-Whitney U-tests.
P
< 0.05 was considered as significant.
Results:
In both unloaded and loaded groups, no statistically significant differences were observed among four composites at the occlusal margin, but a significant difference in gingival microleakage was found between Aelite and silorane. Occlusal and gingival microleakage was not affected by cyclic loading in none of the four restorative materials.
Conclusion:
Silorane did not provide better marginal seal than the low shrinkage methacrylate-based composites (except Aelite). In addition, cyclic loading did not affect the marginal microleakage of evaluated composite restorations .
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Original Article:
The role of vascular endothelial growth factor in proliferation of odontogenic cysts and tumors: An immunohistochemical study
Bhavana Gupta, Shaleen Chandra, Anil Singh, Kunal Sah, Vineet Raj, Vivek Gupta
Dent Res J
2016, 13:256 (10 May 2016)
DOI
:10.4103/1735-3327.182187
PMID
:27274347
Background:
Vascular endothelial growth factor (VEGF) is capable of initiating angiogenesis in blood vessels and may act as mitogenic agent for epithelium of odontogenic cysts and tumors. This study was conducted to evaluate the role of epithelial VEGF expression in odontogenic cysts and ameloblastoma and its correlation with argyrophilic nucleolar organizer region counts to assess its role in their biological behavior.
Materials and Methods:
In this retrospective cross-sectional study, 45 histologically confirmed cases, 15 cases of each of keratocystic odontogenic tumors (KCOTs), dentigerous cysts, and ameloblastomas were examined for immunohistochemical expression for epithelial VEGF, and argyrophilic nucleolar organizer regions (AgNORs) (used as secondary marker in this study) staining was done for comparing the proliferative capacity with VEGF.
Results:
KCOT shows mild expression within the basal layers and strong expression in the suprabasal layer whereas, in dentigerous cysts, a majority showed no VEGF expression whereas ameloblastomas showed strong expression in all cases by stellate reticulum-like cells at the center of the follicles and suprabasal layers of epithelium. The results of AgNOR counts were higher in KCOTs as compared to ameloblastoma and least in dentigerous cysts.
Conclusion:
VEGF expression by the epithelium of odontogenic cysts and tumors may play a role in epithelial proliferation via autocrine mechanism as reflected by increased AgNOR counts. The angiogenic activity via paracrine pathway may be responsible for the difference in growth rate and neoplastic behavior of the lesions.
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Original Article:
The association between periodontal disease parameters and severity of atherosclerosis
Mohammad Ketabi, Fatemeh Rashidi Meybodi, Mohammad Reza Asgari
Dent Res J
2016, 13:250 (10 May 2016)
DOI
:10.4103/1735-3327.182185
PMID
:27274346
Background:
Atherosclerosis is the most common cause for heart attack and stroke. In the last decade, several epidemiological studies have found an association between periodontal infection and atherosclerosis. The aim of this research was to determine the possible association between chronic periodontal disease and severity of atherosclerosis.
Materials
and
Methods:
Eighty-two subjects that were referred to Chamran Heart Hospital in Isfahan for angiography were involved in this study. Fifty-nine subjects had coronary artery obstruction (CAO) and 23 showed no obstruction after angiography. The severity of CAO was assessed. Periodontal parameters including pocket depth (PD), gingival recession (R), clinical attachment level (CAL), and bleeding on probing (BOP) of all subjects were recorded. The decayed-missing-filled (DMF) index of all subjects was also measured. For statistical analysis, Pearson correlation test, Chi-square, and independent
t
-test were used.
Results:
There were significant positive correlation between variables R, PD, CAL, decayed (D), missing (M), DMF, BOP, and degree of CAO. However, there were no significant differences between filling variable degree of CAO (left anterior descending, left circumflex, and right coronary artery). Independent
t
-test showed that the mean of variables R, PD, AL, D, M, and DMF in patients with obstructed arteries were significantly higher than subjects without CAO. But there were no significant differences between variable F in two groups.
Conclusion:
The results of this cross-section analytical study showed an association between periodontal disease and dental parameters with the severity of CAO measured by angiography. However, this association must not interpret as a cause and effect relationship.
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Original Article:
Evaluation of threshold response and appropriate electrode placement site for electric pulp testing in fluorosed anterior teeth: An
in vivo
study
Harikumar Vemisetty, Amulya Vanapatla, Polavarapu Venkata Ravichandra, Surakanti Jayaprada Reddy, Rajani Punna, Srujana Chandragiri
Dent Res J
2016, 13:245 (10 May 2016)
DOI
:10.4103/1735-3327.182184
PMID
:27274345
Background:
Accurate diagnosis is key to success. Diagnosing the pulpal status in varied clinical situations poses a challenge to the clinician. Electric pulp test (EPT) is one of the valuable attempts in evaluating the sensibility of pulp tissue. The aim of this study was to find out and compare the threshold levels and optimal electrode placement site for EPT in fluorosed and nonfluorosed anterior teeth.
Materials and Methodology:
Eighty volunteers recruited for this study were divided into two groups based on the incidence of dental fluorosis. Electric pulp testing was done on either of the central incisors in fluorosed and nonfluorosed group. Four sites on each crown were tested 4 times with digitest electric pulp tester, and the mean of the threshold responses was recorded. The data were analyzed with SPSS, version 11. Means of variables from each location were compared using one-way ANOVA and Tukey's
post-hoc
test while the critical level of significance was set at
P
< 0.05.
Results:
The mean and standard deviation of threshold levels in fluorosed teeth were greater when compared to that of nonfluorosed teeth at all sites with incisal edge showing the least mean threshold levels for both the groups (
P
> 0.05).
Conclusion:
Within the limitations of this study, it can be concluded that fluorosed teeth respond to higher threshold levels than the non-fluorosed teeth, and incisal edge was the optimal electrode placement site.
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Original Article:
Marginal adaptation of Spinell InCeram and feldspathic porcelain laminate veneers
Tahereh Ghaffari, Fahimeh Hamedi-Rad, Vahid Fakhrzadeh
Dent Res J
2016, 13:239 (10 May 2016)
DOI
:10.4103/1735-3327.182183
PMID
:27274344
Background:
This
in vitro
study investigated the marginal fit of two porcelain laminate veneers to help the selection of more accurate veneers in discolored teeth.
Materials and Methods:
Thirty impressions of metal master die created from the prepared labial surface of an acrylic maxillary central incisor were made and poured with Type IV stone. The dies were distributed into test groups (
n
= 15) for the construction of DuCeram and InCeram laminate veneers. An image-analysis program was used to measure the gap between the veneers and the master die at the labial, lingual, and proximal margins. Statistical analysis was performed with repeated measures ANOVA. Independent
t
-test was used to compare the mean values between the two groups. Values of
P
< 0.05 were judged to be significant.
Results:
Differences between marginal fit of two groups were significant (
P
< 0.001). The overall mean marginal gap values (μm) for InCeram and DuCeram were, respectively, 114.4 ± 40.81 and 282.3 ± 82.82. Independent
t
-test revealed significant differences between the marginal gaps of two materials at different predetermined points.
Conclusion:
Within the limitations of this study, the marginal gap in InCeram laminate veneer was within the clinically acceptable standard set at 120 μm.
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Original Article:
A finite element study of teeth restored with post and core: Effect of design, material, and ferrule
Viram Upadhyaya, Akshay Bhargava, Hari Parkash, B Chittaranjan, Vivek Kumar
Dent Res J
2016, 13:233 (10 May 2016)
DOI
:10.4103/1735-3327.182182
PMID
:27274343
Background:
Different postdesigns and materials are available; however, no consensus exists regarding superiority for stress distribution. The aim of this study was to evaluate the effect of design and material of post with or without ferrule on stress distribution using finite element analysis.
Materials and Methods:
A total of 12 three-dimensional (3D) axisymmetric models of postretained central incisors were made: Six with ferrule design and six without it. Three of these six models had tapered posts, and three had parallel posts. The materials tested were titanium post with a composite resin core, nickel chromium cast post and core, and fiber reinforced composite (FRC) post with a composite resin core. The stress analysis was done using ANSYS software. The load of 100 N at an angle of 45 was applied 2 mm cervical to incisal edge on the palatal surface and results were analyzed using 3D von Mises criteria.
Results:
The highest amount of stress was in the cervical region. Overall, the stress in the tapered postsystem was more than the parallel one. FRC post and composite resin core recorded minimal stresses within the post but the stresses transmitted to cervical dentin were more as compared to other systems. Minimal stresses in cervical dentine were observed where the remaining coronal dentin was strengthen by ferrule.
Conclusion:
A rigid material with high modulus of elasticity for post and core system creates most uniform stress distribution pattern. Ferrule provides uniform distribution of stresses and decreases the cervical stresses.
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Original Article:
Effect of local administration of platelet-derived growth factor B on functional recovery of peripheral nerve regeneration: A sciatic nerve transection model
Atefeh Golzadeh, Rahim Mohammadi
Dent Res J
2016, 13:225 (10 May 2016)
DOI
:10.4103/1735-3327.182181
PMID
:27274342
Background:
Effects of platelet-derived growth factor B (PDGF-B) on peripheral nerve regeneration was studied using a rat sciatic nerve transection model.
Materials and Methods:
Forty-five male, white Wistar rats were divided into three experimental groups (
n
= 15), randomly: Normal control group (NC), silicon group (SIL), and PDGF-B treated group (SIL/PDGF). In NC group, left sciatic nerve was exposed through a gluteal muscle incision and after homeostasis muscle was sutured. In the SIL group, the left sciatic nerve was exposed in the same way and transected proximal to tibio-peroneal bifurcation leaving a 10-mm gap. Proximal and distal stumps were each inserted into a silicone conduit and filled with 10 μL phosphate buffered solution. In SIL/PDGF group, the silicon conduit was filled with 10 μL PDGF-B (0.5 ng/mL). Each group was subdivided into three subgroups of five and were studied in 4, 8, 12 weeks after surgery.
Results:
Behavioral testing, sciatic nerve functional study, gastrocnemius muscle mass, and histomorphometric studies showed earlier regeneration of axons in SIL/PDGF than in SIL group (
P
< 0.05).
Conclusion:
Local administration of PDGF-B combined with silicon grafting could accelerate functional recovery and may have clinical implications for the surgical management of patients after facial nerve transection.
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Original Article:
Diagnostic efficacy of color Doppler ultrasound in evaluation of cervical lymphadenopathy
Deepankar Misra, Sapna Panjwani, Shalu Rai, Akansha Misra, Mukul Prabhat, Prashant Gupta, Subrata K Talukder
Dent Res J
2016, 13:217 (10 May 2016)
DOI
:10.4103/1735-3327.182180
PMID
:27274341
Background:
To evaluate the efficacy of color Doppler ultrasound (CDUS) in differentiating benign and malignant cervical lymph nodes by detecting differences in blood flow patterns.
Materials and Methods:
In this cross-sectional prospective study, 25 untreated patients with clinical evidence of cervical lymphadenopathy were evaluated. CDUS was performed for 80 cervical lymph nodes. The gray scale parameters of the lymph node and intranodal perfusion sites were the key CDUS features used to differentiate between reactive and metastatic lymph nodes. Histopathological confirmations were obtained and compared with the results of CDUS.
Results:
Initially, 53 cervical lymph nodes were evaluated by clinical examination. Twenty-seven additional lymph nodes (53 + 27 = 80) were discovered by CDUS evaluation. Gray scale parameters for lymph nodes such as size of lymph node, shape of lymph node, and presence or absence of hilum revealed highly significant results (
P
< 0.0001). Color Doppler flow signals revealed that central/hilar flow was characteristic for benign nodes whereas peripheral/mixed flow was characteristic for malignant nodes, the findings were highly significant (
P
< 0.0001). Gray scale and color Doppler features are used to differentiate benign and malignant nodes.
Conclusion:
Within the limitations of this study, CDUS evaluation was found to be highly significant with a high sensitivity and specificity over clinical evaluation CDUS examination provides a prospect to reduce the need for biopsy/fine needle aspiration cytology in reactive nodes.
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Original Article:
Masticatory ability with depression, anxiety, and stress: Does there exist any association?
Hamidreza Roohafza, Hamid Afshar, Ammar Hassanzadeh Keshteli, Mohamad Javad Shirani, Parastu Afghari, Amrita Vali, Peyman Adibi
Dent Res J
2016, 13:211 (10 May 2016)
DOI
:10.4103/1735-3327.182179
PMID
:27274340
Background:
Masticatory ability as a subjective response to masticatory function indicates patients' perception of their mastication. To the best of our knowledge, there has been no study on association between masticatory ability and psychological status. This study investigated the association between self-assessed masticatory ability (SAMA) and psychological status among a large sample of Iranian adults.
Materials and Methods:
In this cross-sectional study, data were extracted from 4135 adults who had been completed information in 20 counties across Isfahan province regarding masticatory ability and psychological statuses (depression, anxiety, and stress). An SAMA questionnaire, the Hospital Anxiety and Depression Scale and General Health Questionnaire were used to measure masticatory ability, depression and anxiety, and stress of study population. To analyze the data, ANOVA,
χ
2
test, and multinomial regression were applied at 0.05 for the significance level.
Results:
Masticatory ability was significantly correlated with depression (
P
< 0.001), anxiety (
P
< 0.001), and stress (
P
< 0.001). The participants in high masticatory ability group reported lower scores for presence of these psychological variables. Furthermore, males reported significantly higher level for masticatory ability than females (
P
= 0.025). In addition, there were significant association between low and moderate masticatory ability with anxiety (1.09, 1.08), depression (1.15, 1.13), and stress (1.11, 1.09), respectively. The association was still significant after adjusting for age, sex, educational level, and marital status.
Conclusion:
Despite study limitations, the results of this study provide evidence that participants with higher score of depression, anxiety, and stress suffer lower masticatory ability.
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Original Article:
Association between serotonin transporter gene polymorphism and recurrent aphthous stomatitis
Aastha Manchanda, Asha R Iyengar, Seema Patil
Dent Res J
2016, 13:206 (10 May 2016)
DOI
:10.4103/1735-3327.182149
PMID
:27274339
Background:
Anxiety-related traits have been attributed to sequence variability in the genes coding for serotonin transmission in the brain. Two alleles, termed long (L) and short (S) differing by 44 base pairs, are found in a polymorphism identified in the promoter region of serotonin transporter gene. The presence of the short allele and SS and LS genotypes is found to be associated with the reduced expression of this gene decreasing the uptake of serotonin in the brain leading to various anxiety-related traits. Recurrent aphthous stomatitis (RAS) is an oral mucosal disease with varied etiology including the presence of stress, anxiety, and genetic influences. The present study aimed to determine this serotonin transporter gene polymorphism in patients with RAS and compare it with normal individuals.
Materials and Methods:
This study included 20 subjects with various forms of RAS and 20 normal healthy age- and gender-matched individuals. Desquamated oral mucosal cells were collected for DNA extraction and subjected to polymerase chain reaction for studying insertion/deletion in the 5-HTT gene-linked polymorphic region. Cross tabulations followed by Chi-square tests were performed to compare the significance of findings,
P
< 0.05 was considered statistically significant.
Results:
The LS genotype was the most common genotype found in the subjects with aphthous stomatitis (60%) and controls (40%). The total percentage of LS and SS genotypes and the frequency of S allele were found to be higher in the subjects with aphthous stomatitis as compared to the control group although a statistically significant correlation could not be established,
P
= 0.144 and 0.371, respectively.
Conclusion:
Within the limitations of this study, occurrence of RAS was not found to be associated with polymorphic promoter region in serotonin transporter gene.
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Case Report:
Ellis-van Creveld syndrome with unusual oral and dental findings: A rare clinical entity
Sameeulla Shaik, Jayam Raviraj, Suresh Dirasantchu, Suman S Venkata
Dent Res J
2016, 13:193 (8 March 2016)
DOI
:10.4103/1735-3327.178213
PMID
:27076836
Ellis-van Creveld (EVC) syndrome, a form of skeletal and chondroectodermal dysplasia, is an autosomal recessive disorder characterized by a tetrad of disproportionate dwarfism, postaxial polydactyly, ectodermal dysplasia, and heart defects. In the present article, we hereby present a case of a 13-year-old girl of Indian ethnicity with EVC syndrome with a remarkable number of classical oral and dental features, with unusual findings such as taurodontism and talons cusp. Such dental findings were reported in few cases only. Despite the fact that oral manifestations play an important role in the diagnosis of EVC, only a few detailed reports have been published in the dental literature.
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Case Report:
Successful treatment of a large implant periapical lesion that caused paraesthesia and perimandibular abscess
Mohammad Jafarian, Farshid Rayati, Elnaz Najafi
Dent Res J
2016, 13:188 (8 March 2016)
DOI
:10.4103/1735-3327.178211
PMID
:27076835
Successful treatment of a large implant periapical lesion (IPL) that caused paraesthesia and perimandibular abscess. IPL is a pathologic phenomenon that rarely involves implants. This event first described in 1992 with an incidence rate of 0.26-9.9% and the origin is not well known. The most likely suggested causes are presence of preexisting bone pathology, contamination of implant surface, bone overheating during implant surgery, vascular ischemia, excessive tightening of the implant, fenestration of the buccal plate and different implant surface designs. In the present case report, we describe relatively large periapical lesions involving several implants caused severe abscess accompanied by transient inferior alveolar nerve paraesthesia and its successful management. A brief review of the literature and a discussion of possible causes and different treatment plans are also included.
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Original Article:
The effect of washing water temperature on resin-dentin micro-shear bond strength
Mohammad Reza Malekipour, Farzaneh Shirani, Mehrnoush Ebrahimi
Dent Res J
2016, 13:174 (8 March 2016)
DOI
:10.4103/1735-3327.178208
PMID
:27076833
Background:
The purpose of this study was to evaluate the effect of washing water temperature on the micro-shear bond strength (μSBS) of composite resin to dentin using a two-step etch-and-rinse system and a two-step self-etching system.
Materials and Methods:
In this
in vitro
study
,
the intact dentins of buccal and lingual surfaces of healthy third molars were exposed. Dentin surfaces were rinsed with different temperatures of distilled water (20 s) before applying Single Bond (SB) or Clearfil SE Bond(SE). After applying the adhesive, composite cylinders (0.8 mm diameter and 1 mm length) were bonded to the teeth surfaces. After storing the specimens in 37°C distilled water for 48 h and thermocycling, μSBS test was done. Data were analyzed using analysis of variance,
post
hoc
Tukey tests, paired samples
t
-test, and Fisher exact test (α = 0.05).
Results:
Temperature and interaction of temperature and type of bonding agent affected the bond strength. The bond strength of SB groups was significantly higher at 50°C washing than 5°C (
P
= 0.003) and 22°C (
P
= 0.019), but no significant difference was observed between SE groups. The bond strength of SE was significantly higher at 22°C than that of SB (
P
= 0.031), whereas the bond strength of SB was significantly higher at 50°C than that of SE (
P
= 0.007).
Conclusion:
The use of high-temperature washing water is an appropriate method to enhance bond strength in etch-and-rinse systems.
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Original Article:
Antimicrobial efficacy of
Acacia nilotica, Murraya koenigii
(L.)
Sprengel, Eucalyptus
hybrid,
Psidium guajava
extracts and their combination on
Streptococcus mutans
and
Lactobacillus acidophilus
BR Chandra Shekar, Ramesh Nagarajappa, Richa Jain, Rupal Singh, Rupesh Thakur, Suma Shekar
Dent Res J
2016, 13:168 (8 March 2016)
DOI
:10.4103/1735-3327.178206
Background:
The aim of this
in vitro
study was to assess antimicrobial efficacy of
Acacia nilotica, Murraya koenigii
(L.)
Sprengel, Eucalyptus
hybrid
, Psidium guajava
extracts, and their combination on
Streptococcus mutans
and
Lactobacillus acidophilus.
Materials and Methods:
The branches of four plants were collected, identified, and authenticated by a taxonomist. The plants were rinsed in water, healthy leaves were separated and shade dried over a period of 3-4 weeks. Soxhlet apparatus using ethanol was employed for extraction procedure. The combinations of plant extracts were prepared by mixing equal quantities of 10% solutions of each of these extracts. 0.2% chlorhexidine and dimethyl sulfoxide were used as positive and negative controls, respectively. The antimicrobial efficacy testing was done using agar well-diffusion method under anaerobic conditions. The mean diameter of inhibition zone was computed and compared between different categories using one-way analysis of variance and Tukey's
post-hoc
test. A qualitative assay was carried out to identify the various phytochemical constituents in the plants. The data was assessed by SPSS version 20. The statistical significance was fixed at 0.05.
Results:
All the plants extracts and their combinations inhibited
S. mutans
and L. acidophilus
. However, the quadruple combination of
A. nilotica
+
M. koenigii
(L.)
Sprengel
+
Eucalyptus
hybrid +
P. guajava
produced the maximum inhibition zone (23.5 ± 2.2 mm) against
S. mutans
. Although, 0.2% chlorhexidine produced the highest inhibition zone against
L. acidophilus
(18.8 ± 1.2 mm)
, A. nilotica
extract produced maximum inhibition among the various plant extracts and their combinations (14.1 ± 1.8 mm).
Conclusion:
All the individual plant extracts and their combinations were effective against
S. mutans
and
L. acidophilus.
These could be tried as herbal alternates to chlorhexidine. However, these
in vitro
results have to be further evaluated for any toxicity of the polyherbal combinations in animal models and effectiveness has to be assessed using
in vivo
studies on humans.
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Original Article:
Validity and reliability of a Persian version of the quality of masticatory function questionnaire for edentulous patients
Niloufar Khodaeian, Mansour Rismanchian, Ali Behzadi, Fariba Jowkar
Dent Res J
2016, 13:160 (8 March 2016)
DOI
:10.4103/1735-3327.178203
PMID
:27076831
Background:
Questionnaire is a suitable tool for evaluating the subjective masticatory function in edentulous patients. However, there is no validated Persian version of masticatory function questionnaire. The aim of this study was to evaluate the psychometric properties of the translated Persian version of the quality of masticatory function (QMF) questionnaire in terms of validity and reliability.
Materials and Methods:
After translation of QMF questionnaire to Persian, its validity was evaluated by four expert prosthodontists. The tool was applied on 62 complete denture wearers (31 men and 31 women, mean age 64.85 1.98 years, mean time of edentulism 12.17 3.21 years) via face-to-face interviews. Cronbach's alpha coefficient was used to measure internal consistency. Construct validity was analyzed using exploratory factor analysis. The Spearman's correlation coefficient between the summary scores of the tool and the years of edentulism and sex was also calculated for concurrent validity (
α
= 0.05).
Results:
It has been found that the Persian version of the questionnaire had an acceptable reliability (
α
= 0.910). Exploratory factor analysis extracted five domains: Masticatory problems with dentures, problems while consuming apple and carrot, meat products, fruits and vegetables, and changes need for better swallowing. A correlation was found between the tool scores and the years of edentulism (
P
= 0.001), but there was no correlation between sex and the tool scores (
P
= 0.841).
Conclusion:
The Persian version of QMF questionnaire for edentulous patients showed acceptable validity and reliability but further studies are needed to verify this tool.
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Original Article:
A randomized controlled evaluation of alveolar ridge preservation following tooth extraction using deproteinized bovine bone mineral and demineralized freeze-dried bone allograft
Rokhsareh Sadeghi, Maryam Babaei, S Asghar Miremadi, Fatemeh Mashadi Abbas
Dent Res J
2016, 13:151 (8 March 2016)
DOI
:10.4103/1735-3327.178202
PMID
:27076830
Background:
Alveolar ridge preservation could be performed immediately following tooth extraction to limit dimensional changes of alveolar process due to bone resorption. The aim of this study was to compare the clinical and histologic outcomes of socket preservation using two different graft materials; deproteinized bovine bone mineral (DBBM) and demineralized freeze-dried bone allograft (DFDBA) with absorbable collagen membrane.
Materials and Methods:
Twenty extraction sockets in 20 patients were randomly divided into 2 treatment groups: 10 sockets were augmented with DBBM and collagen membrane whereas 10 sockets were filled with DFDBA and covered by collagen membrane. Primary closure was achieved over extraction sockets by flap advancement. Horizontal and vertical ridge dimensional changes were assessed at baseline and after 4-6 months at the time of implant placement. For histological and histomorphometrical analysis, bone samples were harvested from the augmented sites with trephine during implant surgery. All data were analyzed using SPSS version 18 (α=0.05).
Results:
Clinical measurements revealed that average horizontal reduction was 2.3 ± 0.64 mm for DFDBA and 2.26 ± 0.51 mm for DBBM. Mean vertical ridge resorption at buccal side was 1.29 ± 0.68 mm for DFDBA and 1.1 ± 0.17 mm for DBBM. Moreover, mean vertical ridge reduction at lingual site was 0.41 ± 0.38 mm and 0.35 ± 0.34 mm for DFDBA and DBBM, respectively. No significant differences were seen between two groups in any of those clinical parameters. Histologic analysis showed statistically significant more new bone deposition for DFDBA compared to DBBM (34.49 ± 3.19 vs. 18.76 ± 3.54) (
P
< 0.01). Residual graft particles were identified significantly more in DBBM (12.77 ± 1.85) than DFDBA (6.06 ± 1.02).
Conclusion:
Based on the findings of this study, both materials have positive effect on alveolar ridge preservation after tooth extraction, but there was more new bone formation and less residual graft particles in DFDBA group than in DBBM group.
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Original Article:
Prevalence and characteristics of talon cusps in Turkish population
Yeliz Guven, Yelda Kasimoglu, Elif Bahar Tuna, Koray Gencay, Oya Aktoren
Dent Res J
2016, 13:145 (8 March 2016)
DOI
:10.4103/1735-3327.178200
PMID
:27076829
Background:
Talon cusp is a rare dental anomaly characterized by a cusp-like projection, often including the palatal surface of the affected tooth. The aim of the present study was to investigate the prevalence and characteristics of talon cusps in a group of Turkish children.
Materials and Methods:
The study population consisted of 14,400 subjects who attended the clinics of the Department of Pediatric Dentistry at the Istanbul University, Istanbul, Turkey . Subjects ranged in age from 1 to 14 years with a mean age of 10.5 ± 2.55 years. Talon cusps were mainly categorized by visual examination according to the classification of Hattab
et al
. The distribution and frequency of talon cusps were calculated with respect to dentition type, tooth type, talon type, the affected surface, associated dental anomalies, and clinical complications. Statistical analysis included descriptive statistics, frequencies, and crosstabs with Chi-square analysis.
Results:
Talon cusps were detected in 49 subjects (26 males and 23 females) of 14,400 (0.34%). A total of 108 teeth showed talon cusps. Distribution of talon cusps according to gender showed no statistically significant differences. The incidence of talon cusps was found to be greater in maxillary lateral incisors (53.7%) than central incisors (29.62%). Regarding the type of talon cusp, 47.22% of teeth showed a Type III talon cusp, whereas 30.55% of teeth demonstrated a Type II talon and 22.22% of teeth demonstrated a Type I talon cusp. Nine patients (18.36%) with talon cups also exhibited other developmental dental anomalies. Clinical complications associated with talon cusps were detected as caries formation and occlusal interference.
Conclusion:
This is the most comprehensive study of the prevalence of talon cusps in Turkish population using the largest sample size to date. Also, taurodontism associated with a talon cusp has been reported here for the 1
st
time. Clinical complications associated with talon cusps need more investigations.
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Original Article:
Estimation of volume density of interdental papilla components in patients with chronic periodontitis and interleukin-6 (-174G/C) gene polymorphisms
Zahra Heidari, Hamidreza Mahmoudzadeh-Sagheb, Mohammad Hashemi, Somayeh Ansarimoghaddam, Nadia Sheibak
Dent Res J
2016, 13:139 (8 March 2016)
DOI
:10.4103/1735-3327.178199
PMID
:27076828
Background:
The association between interleukin-6 (IL-6) (-174G/C) gene polymorphisms and level of tissue breakdown and periodontal disease progression is unknown. The present study investigated quantitative parameters of interdental papilla in chronic periodontitis (CP) patients with IL-6 (-174G/C) gene polymorphisms.
Materials and Methods:
Sixty gingival samples were studied. After determination of IL-6 (-174G/C) gene polymorphisms using a tetra-primer amplification refractory mutation system-polymerase chain reaction (T-ARMS-PCR) technique, 45 gingival tissue samples of CP patients (GG and GC+CC genotypes) were considered as case groups. Fifteen control samples were also collected from healthy individuals. After tissue processing, interdental gingival tissues were exhaustively sectioned into 4 μm-thick sections. Ten to thirteen sections were sampled by systematic uniform random sampling and stained with Masson trichrome, and the volume density (Vv) of the gingival components was estimated using Cavalier's point counting method. Statistical analysis was performed by Student
t
-test to compare differences between groups. The significance level was set at
P
< 0.05.
Results:
There were statistically significant differences in the Vv of epithelium, connective tissue, collagenous and non-collagenous matrix, and blood vessels between the control and CP groups (
P
< 0.0001). There were no statistically significant differences in the Vv of epithelium, connective tissue of gingiva, collagenous and non-collagenous matrix, and blood vessels among GG, GC, and CC genotypes in CP patients (
P
> 0.05).
Conclusion:
Results of the current study showed that there was no association between IL-6 (-174G/C) gene polymorphisms and quantitative parameters of interdental papilla in CP patients.
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Original Article:
The impact of the global budget system on dynamics of dental manpower and utilization of dental services
Laura Yueh-Guey Huang, Boyen Huang
Dent Res J
2016, 13:132 (8 March 2016)
DOI
:10.4103/1735-3327.178198
PMID
:27076827
Background:
This study aimed to investigate dentists' supply and practice patterns following the implementation of the global budget system in Taiwan.
Materials and Methods:
Data of reimbursement claims, municipal socioeconomic status and dental manpower were collected from the National Health Insurance administration, the Ministry of Internal Affair, and the Ministry of Health and Welfare, respectively. A multivariate linear regression method was used for data analysis.
Results:
A municipality that reported a higher percentage of tertiary educated population (
t
= 3.718,
P
< 0.001), a higher per capita income (
t
= 6.172,
P
< 0.001), a higher population density (
t
= 6.172,
P
< 0.001), or a lower percentage of elderly population (
t
= −2.506,
P
= 0.014) was more likely to have a higher number of dentists per 100,000 population. A municipality that reported a higher Herfindahl-Hirschman Index (HHI) value (
t
= 2.880,
P
= 0.005) was more likely to show a higher move-out rate among dentists. The rate was lower after the implementation of the global budget system (
t
= −2.436,
P
= 0.018). A municipality that had a higher percentage of elderly population (
t
= 3.628,
P
< 0.001), a lower percentage of young population (
t
= −2.138,
P
= 0.035), or a higher rate of population growth (
t
= 4.412,
P
< 0.001) was more likely to display a higher move-in rate among dentists. The percentage of amalgam restorations in total claims reduced from 19.82% to 17.94%, while the percentage of tooth-colored material restorations increased from 25.46% to 28.79%.
Conclusion:
This study has demonstrated a stabilizing effect of the global budget system on dynamics of dental manpower in Taiwan. A relationship between HHI and dentists' move-out rate has been found. The relationship between municipal socioeconomic status and the density of dentists has also been confirmed. In addition, reduced utilization of amalgam restorations was accompanied by increased utilization of tooth-colored material restorations. Further investigations are indicated.
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Original Article:
In-vitro
comparison of the effect of different bonding strategies on the micro-shear bond strength of a silorane-based composite resin to dentin
Pouran Samimi, Vahid Alizadeh, Kamyar Fathpour, Hamid Mazaheri, Vajihosadat Mortazavi
Dent Res J
2016, 13:124 (8 March 2016)
DOI
:10.4103/1735-3327.178197
Background:
The current study evaluated the micro-shear bond strengths of a new low-shrinkage composite resin to dentin.
Materials and Methods:
In this in-vitro study, 70 extracted premolars were assigned to one of seven groups (
n
= 10): Group 1: OptiBond Solo Plus (Opt; Kerr); Group 2: SE Bond (SE; Kuraray); Group 3: Silorane System Adhesive (SSA; 3M ESPE); Group 4: OptiBond Solo Plus + LS Bond (Opt LS); Group 5: SE Bond + LS Bond (SE LS); Group 6: OptiBond Solo Plus (Opt Po); and Group 7: SE Bond (SE Po). Occlusal dentin was exposed and restored with Filtek LS (3M ESPE) in groups 1 to 5 and Point 4 (Kerr) in groups 6 and 7. After thermocycling (1000 cycles at 5/55C), micro-shear bond test was carried out to measure the bond strengths. The results were submitted to analysis of variance and
post
hoc
Tukeytests (
P
< 0.05).
Results:
Two-way ANOVA showed no significant differences between the two types of composite resin (
P
= 0.187), between bonding agents (
P
= 0.06) and between composite resin and bonding agents (
P
= 0.894). Because
P
value of bonding agents was near the significance level, one-way ANOVA was used separately between the two composite groups. This analysis showed significant differences between silorane composite resin groups (
P
= 0.045) and Tukey test showed a significant difference between Groups 4 and 5 (
P
= 0.03).
Conclusion:
The application of total-etch and self-etch methacrylate-based adhesives with and without use of a hydrophobic resin coating resulted in acceptable bond strengths.
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Original Article:
Effect of different adhesive strategies on microtensile bond strength of computer aided design/computer aided manufacturing blocks bonded to dentin
Renato Roperto, Anna Akkus, Ozan Akkus, Lisa Lang, Manoel Damiao Sousa-Neto, Sorin Teich, Thiago Soares Porto
Dent Res J
2016, 13:117 (8 March 2016)
DOI
:10.4103/1735-3327.178196
PMID
:27076825
Background:
The aim of this study was to determine the microtensile bond strength (μTBS) of ceramic and composite computer aided design-computer aided manufacturing (CAD-CAM) blocks bonded to dentin using different adhesive strategies.
Materials and Methods:
In this
in vitro
study, 30 crowns of sound freshly extracted human molars were sectioned horizontally 3 mm above the cementoenamel junction to produce flat dentin surfaces. Ceramic and composite CAD/CAM blocks, size 14, were sectioned into slices of 3 mm thick. Before bonding, CAD/CAM block surfaces were treated according to the manufacturer's instructions. Groups were created based on the adhesive strategy used: Group 1 (GI) - conventional resin cement + total-etch adhesive system, Group 2 (GII) - conventional resin cement + self-etch adhesive system, and Group 3 (GIII) - self-adhesive resin cement with no adhesive. Bonded specimens were stored in 100% humidity for 24h at 37C, and then sectioned with a slow-speed diamond saw to obtain 1 mm × 1 mm × 6 mm microsticks. Microtensile testing was then conducted using a microtensile tester. μTBS values were expressed in MPa and analyzed by one-way ANOVA with
post hoc
(Tukey) test at the 5% significance level.
Results:
Mean values and standard deviations of μTBS (MPa) were 17.68 (±2.71) for GI/ceramic; 17.62 (±3.99) for GI/composite; 13.61 (±6.92) for GII/composite; 12.22 (±4.24) for GII/ceramic; 7.47 (±2.29) for GIII/composite; and 6.48 (±3.10) for GIII/ceramic; ANOVA indicated significant differences among the adhesive modality and block interaction (
P
< 0.05), and no significant differences among blocks only, except between GI and GII/ceramic. Bond strength of GIII was consistently lower (
P
< 0.05) than GI and GII groups, regardless the block used.
Conclusion:
Cementation of CAD/CAM restorations, either composite or ceramic, can be significantly affected by different adhesive strategies used.
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Case Report:
Cone-beam computed tomography as a surgical guide to impacted anterior teeth
Fabiano Jeremias, Camila Maria Bullio Fragelli, Simone Di Salvo Mastrantonio, Lourdes dos Santos-Pinto, Ary dos Santos-Pinto, Cyneu Aguiar Pansani
Dent Res J
2016, 13:85 (22 January 2016)
DOI
:10.4103/1735-3327.174723
PMID
:26962322
Surgical procedure for removal of impacted teeth is a challenge for clinicians as it involves accuracy in the diagnosis and localization of the dental elements. The cone-beam computed tomography (CBCT), compared to the conventional radiography, has a greater potential to provide complementary information because of its three-dimensional (3D) images, reducing the possibility of failures in surgical procedures. Two 10-year-old boys presented with aesthetic issues associated with the juxtaposition of ectopic teeth with the permanent ones. Both two-dimensional and 3D preoperative radiographic diagnostic sets were produced. The occlusal and panoramic radiographs were not enough for proper localization of impacted incisors. Thus, the CBCT was used as a surgical guide. After 2 years of longitudinal following, no lesion was recorded, and the orthodontic treatment has proven successful. In all cases, CBCT contributed to both diagnosis and correct localization of supernumerary teeth, aiding the professional in the treatment planning, and consequently in the clinical success. The surgeries were completely safe, avoiding damage in noble structures, and providing a better recovering of the patients.
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Case Report:
Clinical management of a fused mandibular lateral incisor with supernumerary tooth: A case report
Seda Aydemir, Emre Ozel, Goze Arukaslan, Neslihan Tekce
Dent Res J
2016, 13:80 (22 January 2016)
DOI
:10.4103/1735-3327.174722
PMID
:26962321
The purpose of this report is to present a rare case of a fused mandibular lateral incisor with supernumerary tooth with a follow-up for 18-months. A 35-year-old female patient was referred to our clinic with an extraoral sinus tract in the chin. The intraoral diagnosis revealed the fusion of her mandibular lateral incisors. Vitality pulp tests were negative for mandibular right central and lateral incisors. Radiographic examinations showed a fused tooth with two separate pulp chambers, two distinct roots, and two separate root canals. There were also periapical lesion of fused teeth and mandibular right central incisor, so endodontic treatment was carried out the related teeth. Radiographic examination revealed a complete healing of the lesion postoperatively at the end of 18-months. This paper reports the successful endodontic and restorative treatment of unilateral fused incisors. Because of the abnormal morphology of the crown and the complexity of the root canal system in fused teeth, treatment protocols require special attention.
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Original Article:
Effect of dentifrices on their remineralizing potential in artificial carious lesions: An
in situ
study
Satyawan Gangaramji Damle, Aditi Bector, Dhanashree Damle, Simranjeet Kaur
Dent Res J
2016, 13:74 (22 January 2016)
DOI
:10.4103/1735-3327.174721
PMID
:26962320
Background:
The eventual sequel of dental caries is determined by the dynamic equilibrium between pathological factors which lead to demineralization and protective elements, which in turn leads to remineralization. Remineralization is the natural process for noncavitated demineralized lesions and relies on calcium and phosphate ions assisted by fluoride to rebuild a new surface on existing crystal remnants in subsurface lesions remaining after demineralization. Hence, the present study was designed to evaluate the efficacy of fluoride dentifrices in remineralizing artificial caries-like lesions
in situ
.
Materials and Methods:
A double-blind, randomized study with an initial washout period of 7 days was carried out for 3 weeks. Twenty volunteers were enrolled, who wore the intraoral cariogenicity test appliance having enamel slabs incorporated into them, for 3 weeks. 10 participants were instructed to use Group A dentifrice (fluoride) and the other 10 Group B dentifrice (nonfluoride) for brushing their teeth. The enamel slabs were analyzed by surface microhardness testing and scanning electron microscopy (SEM) at 3 intervals.
Results:
No significant differences was seen in the microhardness values recorded for Group A and Group B at baseline and after demineralization (
P
> 0.05); however Group B exhibited lesser microhardness compared to Group A, after intra-oral exposure (
P
< 0.05). In the SEM analysis, the Group A enamel surfaces had more regular and longer crystallites to those of the Group B.
Conclusion:
Fluoride dentifrices avert the decrease in enamel hardness and loss of minerals from the enamel surface to a large extent as compared to the nonfluoride dentifrices.
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Original Article:
Salivary levels of interleukin-6 and tumor necrosis factor-α in patients undergoing hemodialysis
Faezeh Khozeymeh, Mojgan Mortazavi, Navid Khalighinejad, Mehrdad Akhavankhaleghi, Milad Alikhani
Dent Res J
2016, 13:69 (22 January 2016)
DOI
:10.4103/1735-3327.174720
PMID
:26962319
Background:
Inflammatory cytokines such as interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) are elevated in end-stage renal disease (ESRD). IL-6 and TNF-α are toxins which deteriorate renal function, and their pathogenic role has been confirmed in cardiovascular and oral diseases. This study was designed to investigate the salivary levels of IL-6 and TNF-α in patients with ESRD undergoing hemodialysis (HD). Materials and Methods: Twenty patients with ESRD who were treated with 4 h HD sessions, with low flux membrane were included in this cross-sectional study. Average Kt/V index in patients was 1.19 0.1. Twenty age-sex-matched healthy controls with no infectious diseases during 1 month before saliva sampling were selected. Unstimulated whole saliva was collected and TNF-α and IL-6, concentrations were measured using human IL-6 and TNF-α ELISA kits. Independent
t
-test was used to analyze the data using SPSS (α = 0.05).
Results:
There was a significant difference between dialysis and control groups regarding the salivary levels of TNF-α (
P
= 0.034) and IL-6 (
P
= 0.001).
Conclusion:
Considering the results of this study and reported role of inflammatory cytokines in the pathogenesis of cardiovascular and oral diseases, measurement of salivary IL-6 and TNF-α in HD patients may help in risk stratification of HD patients and in planning pertinent preventive strategies.
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Original Article:
Bispectoral index scores of pediatric patients under dental treatment and recovery conditions: Study of children assigned for general anesthesia under propofol and isofloran regimes
Dana Tahririan, Naser Kaviani, Nosrat Nourbakhsh
Dent Res J
2016, 13:63 (22 January 2016)
DOI
:10.4103/1735-3327.174718
PMID
:26962318
Background:
This study was planned to determine the relationship between bispectoral index (BIS) during dental treatment and recovery conditions in children undergoing two regimes of anesthesia of propofol and isoflurane.
Materials and Methods:
In this single-blind clinical trial study, 57 4-7-year-old healthy children who had been referred for dental treatment under general anesthesia between 60 and 90 min were selected by convenience sampling and assigned to two groups, after obtaining their parents' written consent. The anesthesia was induced by inhalation. For the first group, the anesthesia was preserved by a mixture of oxygen (50%), nitrous oxide (50%), and isoflurane (1%). For the second group, the anesthesia was preserved by a mixture of oxygen (50%), nitrous oxide (50%), and propofol was administered intravenously at a dose of 100 Ng/kg/min. The patients' vital signs, BIS, and agitation scores were recorded every 10 min. The data were analyzed by repeated measure ANOVA and
t
-tests at a significance level of α = 0.05 using SPSS version 20.
Results:
The results of independent
t
-test for anesthesia time showed no statistically significant difference between isoflurane and propofol (
P
= 0.87). Controlling age, the BIS difference between the two anesthetic agents was not significant (
P
> 0.05); however, it was negatively correlated with the duration of anesthesia and the discharge time (
P
= 0.001,
r
= -0.308) and (
P
< 0.001,
r
= -0.55).
Conclusion:
The same depth of anesthesia is produced by propofol and isoflurane, but lower recovery complications from anesthesia are observed with isoflurane.
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Original Article:
Surface microhardness of different thicknesses of a premixed bioceramic material with or without the application of a moist cotton pellet
Noushin Shokouhinejad, Hassan Razmi, Mehrfam Khoshkhounejad, Arezoo Javani, Maryam Raoof
Dent Res J
2016, 13:58 (22 January 2016)
DOI
:10.4103/1735-3327.174715
PMID
:26962317
Background:
This study was conducted to assess the effect of thickness and hydration condition on the surface microhardness of Endosequence Root Repair Material putty (ERRM; Brasseler USA, Savannah, GA), a premixed bioceramic material.
Materials and Methods:
Polymethyl methacrylate cylindrical molds with an internal diameter of 4 mm and three heights of 2, 4, and 6 mm were fabricated. In Group 1 (dry condition), the molds with heights of 2, 4, and 6 mm (10 molds of each) were filled with ERRM. In Groups 2 and 3 (wet condition), a distilled water- or phosphate-buffered saline (PBS)-moistened cotton pellet was placed directly on the upper surface of ERRM, respectively. The lower surface of ERRM was in contact with floral foams soaked with human blood. After 4 days, Vickers microhardness of the upper surface of ERRM was tested. The data were analyzed using two-way analysis of variance. Significance level was set at
P
< 0.05.
Results:
No significant difference was found between the microhardness of three thicknesses of ERRM (2, 4, and 6 mm) with or without placing a distilled water- or PBS-moistened cotton pellet over the material (
P
> 0.05).
Conclusion:
Based on the results of this study, it could be concluded that placing a moistened cotton pellet on ERRM putty up to 6 mm thick might be unnecessary to improve its surface microhardness and hydration characteristics.
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Original Article:
The effect of two remineralizing agents and natural saliva on bleached enamel hardness
Haleh Heshmat, Maryam Hoorizad Ganjkar, Yasaman Miri, Mohamad Javad Kharrazi Fard
Dent Res J
2016, 13:52 (22 January 2016)
DOI
:10.4103/1735-3327.174713
PMID
:26962316
Background:
In order to compensate the adverse consequences of bleaching agents, the use of fluoride-containing remineralizing agents has been suggested by many researchers. The aim of this study was to compare the effect of applying two remineralizing materials on bleached enamel hardness and in comparison to natural saliva.
Materials and Methods:
In this experimental study, 30 enamel samples of sound human permanent molars were prepared for this study. Microhardness (MH) of all specimens was measured and 35% hydrogen peroxide was applied 3 times to the specimens. After completion of the bleaching process, MH of samples was measured and then enamel specimens were divided into three groups each of 10, specimens of groups 1 and 2 were subjected to daily application of hydroxyl apatite (Remin Pro) and casein phosphopeptide amorphous calcium phosphate fluoride (CPP-ACPF) (MI Paste Plus) pastes, respectively, for 15 days. In group 3, the specimens were stored in the operators' natural saliva at room temperature in this period of time. Final MH of all groups was measured. The data were analyzed using repeated measures ANOVA (α = 0.05).
Results:
The hardness significantly decreased in all groups following bleaching. Application of either Remin Pro, CPP-ACPF or natural saliva increased the hardness significantly. The hardness of the three test groups after 15 days were statistically similar to each other.
Conclusion:
The hardness of enamel increases eventually after exposure to either MI Paste Plus, Remin Pro or natural saliva.
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Original Article:
Evaluation of biofilm removal activity of
Quercus infectoria
galls against
Streptococcus mutans
Maryam Mohammadi-Sichani, Vajihe Karbasizadeh, Samaneh Chaharmiri Dokhaharani
Dent Res J
2016, 13:46 (22 January 2016)
DOI
:10.4103/1735-3327.174708
PMID
:26962315
Background:
Dental caries is one of the most prevalent infectious diseases affecting humans of all ages.
Streptococcus mutans
has an important role in the development of dental caries by acid production. The purpose of this study was to evaluate the antibacterial and biofilm disinfective effects of the oak tree
Quercus infectoria
galls against
S. mutans
.
Materials and Methods:
The bacterial strain used in this study was
S. mutans
(ATCC: 35668). Two kinds of galls, Mazouj and Ghalghaf were examined. Galls were extracted by methanol, ethanol and acetone by Soxhlet apparatus, separately. Extracts were dissolved in sterile distilled water to a final concentration of 10.00, 5.00, 2.50, 1.25, 0.63, 0.31, and 0.16 mg/ml. Microdilution determined antibacterial activities. The biofilm removal activities of the extracts were examined using crystal violet-stained microtiter plate method. One-way ANOVA was used to compare biofilm formation in the presence or absence of the extracts.
Results:
The methanolic, ethanolic, and acetonic extracts of
Q. infectoria
galls showed the strong inhibitory effects on
S. mutans
(
P
< 0.05). The minimum inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) values for the Mazouj and Ghalghaf gall extracts against
S. mutans
were identical. The MIC values ranged from 160 μg/ml to 320 μg/ml, whereas the MBC values ranged from 320 μg/ml to 640 μg/ml. All extracts of
Q. infectoria
galls significantly (
P
< 0.05) reduced biofilm biomass of
S. mutans
at the concentrations higher than 9.8 μg/ml.
Conclusion:
Three different extracts of
Q. infectoria
galls were similar in their antibacterial activity against
S. mutans
. These extracts had the highest biofilm removal activities at 312.5 μg/ml concentration. The galls of
Q. infectoria
are potentially good sources of antibacterial and biofilm disinfection agent.
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Original Article:
Prediction of lip response to orthodontic treatment using a multivariable regression model
Amin Shirvani, Saeid Sadeghian, Safieh Abbasi
Dent Res J
2016, 13:38 (22 January 2016)
DOI
:10.4103/1735-3327.174697
PMID
:26962314
Background:
This was a retrospective cephalometric study to develop a more precise estimation of soft tissue changes related to underlying tooth movment than simple relatioship betweenhard and soft tissues.
Materials and Methods:
The lateral cephalograms of 61 adult patients undergoing orthodontic treatment (31 = premolar extraction, 31 = nonextraction) were obtained, scanned and digitized before and immediately after the end of treatment. Hard and soft tissues, angular and linear measures were calculated by Viewbox 4.0 software. The changes of the values were analyzed using paired
t
-test. The accuracy of predictions of soft tissue changes were compared with two methods: (1) Use of ratios of the means of soft tissue to hard tissue changes (Viewbox 4.0 Software), (2) use of stepwise multivariable regression analysis to create prediction equations for soft tissue changes at superior labial sulcus, labrale superius, stomion superius, inferior labial sulcus, labrale inferius, stomion inferius (all on a horizontal plane).
Results:
Stepwise multiple regressions to predict lip movements showed strong relations for the upper lip (adjusted
R
2
= 0.92) and the lower lip (adjusted
R
2
= 0.91) in the extraction group. Regression analysis showed slightly weaker relations in the nonextraction group.
Conclusion:
Within the limitation of this study, multiple regression technique was slightly more accurate than the ratio of mean prediction (Viewbox4.0 software) and appears to be useful in the prediction of soft tissue changes. As the variability of the predicted individual outcome seems to be relatively high, caution should be taken in predicting hard and soft tissue positional changes.
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Original Article:
Microbiological analysis after complete or partial removal of carious dentin using two different techniques in primary teeth: A randomized clinical trial
Deepak Kumar Singhal, Shashidhar Acharya, Arun Singh Thakur
Dent Res J
2016, 13:30 (22 January 2016)
DOI
:10.4103/1735-3327.174695
PMID
:26962313
Background:
The management of deep carious lesions can be done by various techniques but residual caries dilemma still persists and bacterial reduction in cavities treated by either partial or complete caries removal techniques is debatable. So the objective of the present randomized clinical trial was to compare microbial counts in cavities submitted to complete caries removal and partial caries removal using either hand instruments or burs before and after 3 weeks of restoration.
Materials and Methods:
Primary molars with acute carious lesions in inner half of dentine and vital pulp were randomly divided into three groups of 14 each: Group A: Partial caries removal using hand instruments atraumatic restorative treatment (ART) only; Group B: Partial caries removal using bur; Group C: Complete caries removal using bur and caries detector dye. Dentine sample obtained after caries removal and 3 weeks after restoration, were subjected to microbial culture and counting (colony-forming units [CFU]/mg of dentine) for total viable bacterial count,
Streptococcus
spp., mutans streptococci,
Lactobacillus
spp.
Results:
Three techniques of caries removal showed significant (
P
< 0.05) reduction in all microorganisms studied after 3 weeks of evaluation, but there was no statistically significant difference in percentage reduction of microbial count among three groups.
Conclusion:
Results suggest the use of partial caries removal in a single session as compared to complete caries removal as a part of treatment of deep lesions in deciduous teeth in order to reduce the risk of pulp exposure. Partial caries removal using ART can be preferred for community settings as public health procedure for caries management.
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Original Article:
The effect of low-level helium-neon laser on oral wound healing
Farimah Sardari, Farzaneh Ahrari
Dent Res J
2016, 13:24 (22 January 2016)
DOI
:10.4103/1735-3327.174693
PMID
:26962312
Background:
The effectiveness of low power lasers on incisional wound healing, because of conflicting results of previous studies, is uncertain. Therefore, the aim of this study was to evaluate the effects of low-level helium-neon (He-Ne) laser irradiation on wound healing in rat's oral mucosa.
Materials and Methods:
Sixty-four standardized incisions were carried out on the buccal mucosa of 32 male Wistar divided into four groups of eight animals each. Each rat received two incisions on the opposite sides of the buccal mucosa by a steel scalpel. On the right side (test side), a He-Ne laser (632 nm) was employed on the incision for 40 s. Laser radiation was used just in 1
st
day, 1
st
and 2
nd
day, 1
st
and 3
rd
day, and continuous 3 days in groups of A, B, C, and D of rats, respectively. The left side (control side) did not receive any laser. Histological processing and hematoxylin and eosin staining were done on tissue samples after 5 days. Wilcoxon and Kruskal-Wallis tests were used for statistical analysis.
Results:
Histological analysis showed that the tissue healing after continuous 3 days on the laser irradiated side was better than the control side, but there was no difference between the two sides in each groups (
P
> 0.05).
Conclusion:
This study showed that He-Ne laser had no beneficial effects on incisional oral wound healing particularly in 5 days after laser therapy. Future research in the field of laser effects on oral wound healing in human is recommended.
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Original Article:
Plasma levels of N-telopeptide of Type I collagen in periodontal health, disease and after treatment
Ganganna Aruna
Dent Res J
2016, 13:18 (22 January 2016)
DOI
:10.4103/1735-3327.174691
PMID
:26962311
Background:
To determine plasma concentrations of bone resorption marker cross-linked N-terminal telopeptide (NTx) of Type I collagen in periodontal health, disease and after nonsurgical periodontal therapy in chronic periodontitis group.In addition, to know the association between plasma NTx levels and the different clinical parameters.
Materials and Methods:
Thirty subjects were divided on the basis of their periodontal status and were categorized as Group I: Healthy, Group II: Gingivitis, and Group III: Chronic periodontitis. Group III subjects were treated with scaling and root planing, 6-8 weeks later blood samples were analyzed, and they constituted Group IV. NTx levels in plasma were analyzed by competitive - enzyme-linked immunosorbent assay. All data were analyzed using statistical software (SPSS) (α = 0.05).
Results:
All the samples tested positive for the presence of NTx. The mean NTx concentration was highest in Group III (18.77 nanomole Bone Collagen Equivalent [nm BCE]) and the lowest in Group IV (16.02 nm BCE). The values of Group I and Group II fell between the highest and the lowest values (16.23 nm BCE and 16.70 nm BCE, respectively).The difference in mean NTx levels in Group III and Group IV were statistically significant. NTx levels in all the groups positively correlated with the clinical parameters. All data were analyzed using statistical software (SPSS) (α = 0.05).
Conclusion:
Within the limits of this study, it may be suggested that plasma NTx levels may provide distinguishing data between periodontally healthy diseased sites and after nonsurgical therapy of diseased sites.
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Online since 26
th
February, 2015